Fig 1: HBA reduces mitochondrial Apaf-1/caspase-9 pathway-mediated caspase-3 activation induced by 6-OHDA. SH-SY5Y cells were pretreated with HBA (120 µmol/L), NALC (1 mmol/L), or SP600125 (10 µmol/L) for 1 h and then exposed to 6-OHDA (100 μmol/L) for another 24 h. (A) Western blot was carried out to detect the expression of Apaf-1, cleaved caspase-9 (Casp 9), cleaved caspase-3 (Casp 3), and cleaved PARP-1. Band densities of proteins have been normalized to β-actin. (B) Caspase-9, caspase-3, and PARP activities were evaluated using the commercial colorimetric assay kits. The results are shown as the mean ± SD of five independent experiments (n = 5) performed in triplicate. a p < 0.05 and b p < 0.01 compared to the data from untreated control group (control); c p < 0.05 and d p < 0.01 compared to the data from cells exposed to 6-OHDA with no treatment.
Fig 2: Hispidin reduces caspase-mediated apoptosis induced by MPP+. MS23.5 cells were pretreated with hispidin (20 µmol/L), JNK-IN-8 (1 µmol/L), or SR3576 (25 µmol/L) for 1 h and then exposed to 2 mmol/L MPP+ for another 24 h. (A) Caspase-9, caspase-3, and PARP activities were assessed using commercial colorimetric assay kits. (B) The extent of apoptosis was quantified using the ELISA kit to detect DNA fragments associated with cytoplasmic histones. The results are shown as the mean ± SD of five independent experiments (n = 5) performed in triplicate. a p < 0.05 and b p < 0.01 compared to the untreated control group. c p < 0.05 and d p < 0.01 compared to the untreated MPP+ group.
Supplier Page from R&D Systems, a Bio-Techne Brand for PARP/Apoptosis Colorimetric Assay Kit