EnGensgRNA Synthesis Kit,S. pyogenes E3322V from New England Biolabs

Product Specs
ItemEnGensgRNA Synthesis Kit,S. pyogenes
CompanyNew England Biolabs
Price $232.00 Inquire
Catalog NumberE3322V
Quantity10 reactions
ApplicationsFunctional Genomics
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

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Website: http://www.neb.com

(1) Cytosolic localization of MBL-1/Muscleblind may be required for ectopic neurite outgrowth in a sensitized background in C. elegansMicroPubl BiolMay 7, 2026Ho Ming Terence Lee, Chaogu ZhengSpecifically, pairs of single guide RNAs (sgRNAs) were synthesized using the EnGen sgRNA Synthesis Kit (NEB, E3322V). A total of 1 μg of each sgRNA pair, combined with 20 pmol of recombinant Cas9 protein (EnGen S. pyogenes

(2) CHK1 is an integral regulator of DNA replication in human cellsCell Death & DiseaseMarch 26, 2026Siting Li, Dandan Zhu, Mengfan Tang, Min Huang, Xu Feng, Litong Nie, Huimin Zhang, Ling Yin, Sarah Keast, Chang Yang, et al.PCR. sgRNAs were synthesized using the EnGen® sgRNA Synthesis Kit, S. pyogenes (New England Biolabs, E3322V/S). Briefly, EnGen 2× sgRNA Reaction Mix, target-specific DNA oligonucleotide (1 μM), DTT (0.1 M), and

(3) Imaging the time course of DNA damage response at a nonrepetitive endogenous locusCell Reports MethodsNovember 17, 2025Adam T. Rybczynski, W. Taylor Cottle, Po-Ta Chen, Jiwoong Kwon, Tiantian Shang, Yanbo Wang, Paul Meneses, Sushil Pangeni, Yeji Park, Momcilo Gavrilov, et al.the ACTB gene were synthesized in vitro as a pooled mixture using the EnGen RNA Synthesis Kit (NEB, E3322V), following the manufacturer’s instructions. The resulting RNA was purified using the Monarch RNA Cleanup

(4) The First CRISPR-Based Therapeutic (SL_1.52) for African Swine Fever Is Effective in SwineVirusesNovember 14, 2025Naveen Verma, Alison O’Mahony, Roky Mohammad, Dylan Keiser, Craig W. Mosman, Deric Holden, Kristin Starr, Jared Bauer, Bradley Bauer, Roypim Suntisukwattana, et al.Sequences First, single guide RNA (sgRNA) was synthesized in vitro using EnGen® sgRNA Synthesis Kit (E3322V) from New England Biolabs (NEB, Ipswich, MA, USA). Briefly, the EnGen 2X sgRNA Reaction Mix, along with

(5) Maternal effects on postembryonic neuroblast migration in Caenorhabditis elegansG3: Genes, Genomes, GeneticsSeptember 3, 2025Hoikiu Poon, Chaogu Zhengrespectively. The single guide RNAs (sgRNAs) were synthesized using the NEB EnGen sgRNA Synthesis Kit (E3322V) and were injected together with recombinant Cas9 (EnGen S. pyogenes Cas9 NLS from NEB, M0646T) into

(6) CACNA1G, A Heterotaxy Candidate Gene, Plays a Role in Ciliogenesis and Left‐Right Patterning in Xenopus tropicalisgenesisFebruary 1, 2025Valentyna Kostiuk, Rakib Kabir, Rashid Akbari, Amy Rushing, Delfina P. González, Angelina Kim, Ashley Kim, David Zenisek, Mustafa K. Khokhawe designed sgRNA oligos using CRISPRscan (CRISPRscan.org). The EnGen sgRNA Synthesis Kit (Catalog # E3322V, New England Biolabs) and Zymo RNA clean and concentrator kit (Catalog # R1019, Zymo Research) were

(7) G-quadruplex stabilization provokes DNA breaks in human PKD1, revealing a second hit mechanism for ADPKDNat CommunJanuary 2, 2025Agata M. Parsons, Seth Byrne, Jesse Kooistra, John Dewey, Aaron L. Zebolsky, Gloria Alvarado, Gerrit J. Bouma, Gregory B. Vanden Heuvel, Erik D. LarsonSNAP-tagged dCAS9, TMR-snap ligand, and sgRNA synthesis kit (New England Biolabs, #M0652, #S9105S, #E3322V) were used as reagents, and since tween disrupted tissue morphology PBS alone was use in washes. 94

(8) MBL-1/Muscleblind regulates neuronal differentiation and controls the splicing of a terminal selector in Caenorhabditis elegansPLOS GeneticsOctober 18, 2024Ho Ming Terence Lee, Hui Yuan Lim, Haoming He, Chun Yin Lau, Chaogu ZhengSpecifically, a pair of single guide RNAs (sgRNAs) were synthesized using the EnGen sgRNA Synthesis Kit (E3322V) from NEB; a total of 1μg of the sgRNA pair along with 20 pmol recombinant Cas9 (EnGen S. pyogenes Cas9

(9) A syngeneic spontaneous zebrafish model of tp53-deficient, EGFRvIII, and PI3KCAH1047R-driven glioblastoma reveals inhibitory roles for inflammation during tumor initiation and relapse in vivoElifeJuly 25, 2024Alex Weiss, Cassandra D'Amata, Bret J Pearson, Madeline N Hayesal., 2019). gRNAs were in vitro synthesized using the EnGen sgRNA Synthesis Kit (New England Biolabs, E3322V) according to the manufacturer’s recommendation. Cas9 protein with NLS (PNA Bio, CP01-200) was resuspended

(10) A syngeneic spontaneous zebrafish model of tp53-deficient, EGFRvIII, and PI3KCAH1047R-driven glioblastoma reveals inhibitory roles for inflammation during tumor initiation and relapse in vivoeLifeJuly 25, 2024Alex Weiss, Cassandra D'Amata, Bret J Pearson, Madeline N Hayesal., 2019). gRNAs were in vitro synthesized using the EnGen sgRNA Synthesis Kit (New England Biolabs, E3322V) according to the manufacturer’s recommendation. Cas9 protein with NLS (PNA Bio, CP01-200) was resuspended

(11) A method for generating user‐defined circular single‐stranded DNA from plasmid DNA using Golden Gate intramolecular ligationBiotechnology and BioengineeringOctober 1, 2023Isabell K. Strawn, Paul J. Steiner, Matilda S. Newton, Zachary T. Baumer, Timothy A. WhiteheadCleavage Experiments To make the sgRNA, the EnGen sgRNA Synthesis Kit, S. pyogenes (NEB; catalog # E3322V) was used with a DNA primer as a template (Supplementary Table 1). The linearization of the cssDNA was

(12) Lactucin Synthase Inactivation Boosts the Accumulation of Anti-inflammatory 8-Deoxylactucin and Its Derivatives in Chicory (Cichorium intybus L.)Journal of agricultural and food chemistryApril 10, 2023Cankar K, Hakkert JC, Sevenier R, Papastolopoulou C, Schipper B, Baixinho JP, Fernández N, Matos MS, Serra AT, Santos CN, et al.obtained from New England Biolabs, and the guides were produced using the EnGen sgRNA synthesis kit (E3322V) following the manufacturer’s instructions. The transfection of the CRISPR/Cas9 reagents, regeneration

(13) Achieving single nucleotide sensitivity in direct hybridization genome imagingNature CommunicationsDecember 15, 2022Yanbo Wang, W. Taylor Cottle, Haobo Wang, Momcilo Gavrilov, Roger S. Zou, Minh-Tam Pham, Srinivasan Yegnasubramanian, Scott Bailey, Taekjip Haused in this study, the guide RNA was synthesized using EnGen® sgRNA Synthesis Kit, S. pyogenes (NEB, E3322V) according to the manufacturer’s instructions. The template DNA sequences are listed in Supplementary

(14) Polyvalent guide RNAs for CRISPR antiviralsiScienceNovember 18, 2022Rammyani Bagchi, Rachel Tinker-Kulberg, Mohammad Salehin, Tinku Supakar, Sydney Chamberlain, Ayalew Ligaba-Osena, Eric A. Josephsrecombinant proteins" ,, Sodium carbonate,Fluka,Cat#71350 EnGen RNA synthesis kit,New England Biolabs,Cat#E3322V Monarch RNA cleanup kit,New England Biolabs,Cat#T2040S Universal Human Reference RNA,Invitrogen,Cat#QS0639 Human Lung Total RNA

(15) STRAIGHT-IN enables high-throughput targeting of large DNA payloads in human pluripotent stem cellsCell Rep MethodsOctober 24, 2022Blanch-Asensio A, Grandela C, Brandão KO, de Korte T, Mei H, Ariyurek Y, Yiangou L, Mol MPH, van Meer BJ, Kloet SL, et al.High Pure PCR Template Preparation Kit,Roche,Cat#11796828001 EnGen® sgRNA Synthesis Kit,New England Biolabs,Cat#E3322V PCR product using the Wizard® SV Gel and PCR Clean-Up System kit,Promega,Cat#A9281 NucleoBond® Xtra Midi

(16) CRISPR/Cas9 targeted inactivation of the kauniolide synthase in chicory results in accumulation of costunolide and its conjugates in taprootsFrontiers in Plant ScienceAugust 29, 2022Katarina Cankar, Johanna Christina Hakkert, Robert Sevenier, Eva Campo, Bert Schipper, Christina Papastolopoulou, Khabat Vahabi, Alain Tissier, Paul Bundock, Dirk Boschobtained from New England Biolabs and the KLSp1 guide was produced using the EnGen sgRNA synthesis kit (E3322V) following the manufacturer’s instructions. Isolation and transfection of chicory protoplasts Cichorium intybus

(17) Reduced Expression of TMEM16A Impairs Nitric Oxide-Dependent Cl− Transport in Retinal Amacrine CellsFrontiers in Cellular NeuroscienceJuly 27, 2022Tyler Christopher Rodriguez, Li Zhong, Hailey Simpson, Evanna Gleasonordered from IDT, and transcription was performed according to the EnGen sgRNA Synthesis Kit (NEB, E3322V). Target DNA was PCR-amplified from E18 chick retina genomic DNA. We then assessed each guide RNA for

(18) CRISPR-Cas12a ribonucleoprotein-mediated gene editing in the plant pathogenic fungus Magnaporthe oryzaeSTAR ProtocolsMarch 18, 2022Jun Huang, David E. CookSynthesis Kit for the S. pyogenes SpCas9 protein can be used for making the corresponding gRNA (NEB, Cat#E3322V).CRITICAL: If high-quality genome assembly of your isolate is not available, you can check the potential

(19) Inactivation of the germacrene A synthase genes by CRISPR/Cas9 eliminates the biosynthesis of sesquiterpene lactones in Cichorium intybus LPlant Biotechnology JournalDecember 1, 2021Katarina Cankar, Paul Bundock, Robert Sevenier, Suvi T. Häkkinen, Johanna Christina Hakkert, Jules Beekwilder, Ingrid M. van der Meer, Michiel de Both, Dirk BoschsgRNA1 was generated using an oligonucleotide and the EnGen sgRNA Synthesis kit (New England Biolabs, E3322V). The protected version of sgRNA1 contained three phosphorothioate linkages and 2’‐O‐methyl RNA modifications

(20) Epistatic, synthetic, and balancing interactions among tubulin missense mutations affecting neurite growth in Caenorhabditis elegansMolecular Biology of the CellFebruary 15, 2021Ho Ming Terence Lee, Natalie Yvonne Sayegh, A. Sophia Gayek, Susan Laura Javier Jao, Martin Chalfie, Chaogu ZhengsgRNAs targeting specific sequences in mec-7 were synthesized using the EnGen sgRNA Synthesis Kit (E3322V) from New England Biolabs (NEB; Ipswich, MA) and purified using NEB Monarch RNA Cleanup Kit (T2030L);

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EnGensgRNA Synthesis Kit,S. pyogenes

EnGensgRNA Synthesis Kit,S. pyogenes from New England Biolabs

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EnGensgRNA Synthesis Kit,S. pyogenes from New England Biolabs

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