NEBNext rRNA Depletion Kit (Bacteria) E7850X from New England Biolabs

Product Specs
ItemNEBNext rRNA Depletion Kit (Bacteria)
CompanyNew England Biolabs
Price $3,963.00 Inquire
Catalog NumberE7850X
Quantity96 reactions
ApplicationsDNA Analysis, NGS
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) A Bait-and-Switch Strategy Links Phenotypes to Genes Coding for Polymer-Degrading Enzymes in Intact MicrobiomesMicrob BiotechnolApril 26, 2026Colleen E. Yancey, Kyle D. Brumfield, Jackson A. Buss, Rita R. Colwell, Laurence Ettwillerlibrary preparation. Ribosomal depletion was completed using the NEBNext rRNA Depletion Kit for Bacteria (E7850, New England Biolabs, Ipswich, MA, USA) according to the manufacturer's instructions. Libraries with

(2) Genetic analysis of Mycobacterium abscessus reveals genomic diversity linked to global plasmid distributioniScienceApril 17, 2026Kensuke Ohse, Atsushi Yoshida, Keisuke Kamada, Hironobu Kitazawa, Yusuke Ito, Takayo Shoji, Kenichiro Watanabe, Hiroshi Koganemaru, Ken Kikuchi, Masashi Toyoda(R9.4.1),Oxford Nanopore Technologies,Cat.# FLO-MIN106 NEBNext rRNA Depletion Kit (Bacteria),NEB,Cat.# E7850 ,, Deposited data,Deposited data,Deposited data ,, All raw sequence data generated in this study,DDBJ,BioProject:

(3) The Landscape and Regulatory Determinants of A-to-I RNA Editing in Escherichia coli and Pseudomonas aeruginosa Isolated From Patients With Urinary Tract and Ear InfectionsThe Journal of Infectious DiseasesMarch 17, 2026Eyal Elias, Danielle Keidar-Friedman, Nadav Sorek, Orit Raz, Sharon Ovnat Tamir, Liam Aspit, Dan Bar YaacovRNA-seq Ribosomal RNA was depleted using NEBNext® rRNA Depletion Kit (Bacteria) (New England Biolabs, # E7850). Libraries were constructed using NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina®

(4) Microbial Community multi-omic analysis of marsh sediment post crustacean shell compost enrichment: pathogen emergence and community responsebioRxivMarch 6, 2026Colleen E. Yancey, Kyle D. Brumfield, Laurence Ettwiller, Rita R. Colwellwas completed using NEBNext® rRNA Depletion Kit for Bacteria (New England Biolabs, Ipswich, MA, USA, E7850), following the manufacturer instructions. Libraries with ~200 bp inserts were prepared with NEBNext®

(5) LncRNA SLC16A1-AS1 cooperates with NSUN2 to stabilize GRP78 mRNA via m5C modification in gastric canceriScienceFebruary 20, 2026Beiyao Zheng, Wenjing Zhao, Yan Jin, Wentao Sun, Yong Li, Qi Zhang, Yue Li, Jin Wang, Dong Chen, Ke Ren, et al.Scientific,Cat#A55864 ClonExpress Ultra One Step Cloning Kit V2,Vazyme,Cat#C116-01 NEBNext® rRNA Depletion Kit,NEB,Cat#E7850L ,, Biological samples,Biological samples,Biological samples ,, Human GC tissues,Nanjing First Hospital,This

(6) Aerobicity stimulon in Escherichia coli revealed using multi-scale computational systems biology of respiratory variantsiScienceFebruary 20, 2026Arjun Patel, Neha Banwani, Raegan Mink, Divya M. Prabhakaran, Snehal V. Khairnar, Adam M. Feist, Bernhard O. Palsson, Amitesh Anandscreen tape. Ribosomal RNA was removed using the NEBNext rRNA depletion kit for bacteria (Cat. no. NEB E7850X), and double-stranded cDNA libraries were constructed using the NEBNext Ultra II Directional RNA Library

(7) Norepinephrine derepresses the Fur regulon of Neisseria gonorrhoeae to enable growth in iron-limited conditionsJournal of BacteriologyFebruary 19, 2026Camille S. Westlake, Julie L. Stoudenmire, Indu Bhatia, Yasiru R. Perera, Rachel M. Krueger, Cynthia Nau Cornelissen, Walter J. Chazin, Melissa M. Kendall, Alison K. CrissUniversity of Maryland. rRNA reduction was completed using NEBNext rRNA Depletion Kit (Bacteria) (NEB E7850), and RNA library prep was done using NEBNext Ultra II Directional RNA Library Prep Kit (NEB E7760).

(8) Dietary fat disrupts a commensal-host lipid network that promotes metabolic healthCell MetabolismJanuary 6, 2026Kendra Klag, Darci Ott, Trevor S. Tippetts, Rebekah J. Nicolson, Sean M. Tatum, Kaylyn M. Bauer, Emmanuel Stephen-Victor, Allison M. Weis, Rickesha Bell, James Weagley, et al.SML1286 Myriocin,Sigma-Aldrich,Cat# M1177-25MG NEBNext rRNA Depletion Solution (bacteria),NEB,Cat# E7850L OCT,Fisher,Cat# 23-730.571 Palmitic acid,Sigma-Aldrich,Cat# P5585 Palmitic acid alkyne,Cayman Chemical,Cat#

(9) Structural-guided identification of two modulators of β−1,3-glucan synthase FKS1Nature CommunicationsDecember 7, 2025Jialu Li, Jian Li, Angqi Zhu, Xinli Dai, Juxiu Liu, Huayi Liu, Zhili Xia, Yuefan Dong, Wenyu Qian, Lunzhi Dai, et al.using NEBNext® Multiplex Small RNA Library Prep Set for Illumina® kit (New England Biolabs, E7300L/E7850L) and sequenced by Illumina NextSeq 500 system. The quality control of sequencing data was performed

(10) Expression of dnaE2 promotes genetic diversity in mycobacterial biofilmsFront Cell Infect MicrobiolDecember 1, 2025S. Salini, Sinchana G. Bhat, M. Nijisha, Kumar Paritosh, Reshma Ramachandran, Nikhil Nikolas, Saba Naz, Krishna Kurthkotisamples in each group) was depleted of bacterial rRNA using the NEBNext rRNA Depletion Kit (Cat. No. E7850X; New England Biolabs, Ipswich, MA, USA) according to the manufacturer’s protocol, which included a DNase

(11) A Bait-and-Switch strategy links phenotypes to genes coding for Polymer-Degrading Enzymes in Intact MicrobiomesbioRxivOctober 14, 2025Colleen E. Yancey, Kyle D. Brumfield, Jackson Buss, Rita R. Colwell, Laurence Ettwillerpreparation. Ribosomal depletion was completed using the NEBNext® rRNA Depletion Kit for Bacteria (E7850, New England Biolabs, Ipswich, MA, USA) according to the manufacturer's instructions. Libraries with

(12) Whole-genome sequencing of Lassa virus from dry blood spots: a comparative evaluationInfect Dis PovertyOctober 13, 2025Umaru Bangura, Christopher Davis, Andreas Dahl, Sylvia Klemroth, Emma Thomson, N 'Faly Magassouba, Elisabeth Fichet-CalvetNEBNext rRNA Depletion Kit v2 (human/mouse/rat; New England Biolabs, E7400X) and the NEBNext rRNA Depletion Kit (Bacteria, New England Biolabs, E7850X). Sequencing was performed on the Illumina NovaSeq 6000 using the NEBNext Ultra II RNA Library Prep

(13) Human gut bacteria produce structurally related monoglycolipids with contrasting immune functionsNature MicrobiologyOctober 10, 2025Ji-Sun Yoo, Da-Jung Jung, Byoungsook Goh, Kyoo Heo, Wen Zheng, ChangWon C. Lee, Jeong In Seo, Naama Geva-Zatorsky, Meng Wu, Seung Bum Park, et al.confirmed by PCR of 16S rRNA DNA region. mRNA was enriched using a rRNA Depletion Kit (Bacteria) (NEB E7850). The sequencing library was constructed with Ultra™ II Directional RNA Library Prep with Sample Purification

(14) Stool shed cell transcriptomics mirrors tumor biology and enables colorectal cancer diagnosisSci RepOctober 2, 2025Keren Bahar Halpern, Ilan Kent, Oran Yakubovsky, Shani Ben-Moshe, Tal Barkai, Michal Fine, Roy Novoselsky, Alon Israeli, Ido Nachmany, Shalev Itzkovitzdepleted from bacterial ribosomal RNA using the NEBNext rRNA Depletion Kit (Bacteria, New England Biolabs, E7850X) according to the manufacturer instruction. Briefly: one third of total isolated stool RNA was used

(15) Bacterial RNA promotes proteostasis through inter-tissue communication in C. elegansNat CommunOctober 1, 2025Emmanouil Kyriakakis, Chiara Medde, Danilo Ritz, Geoffrey Fucile, Alexander Schmidt, Anne Spangdepletion was performed on 300 ng E. coli total RNA using NEBNext rRNA Depletion Kit Bacteria (Cat#E7850L, NEB, Ipswich, MA, USA). Following elution in 8 µl water, 1 µl of eluate for monitoring the depletion

(16) A-to-I mRNA editing recodes hundreds of genes in dozens of species and produces endogenous protein isoforms in bacteriaNucleic Acids ResJuly 12, 2025Eyal Elias, Isaac Gifford, Liron Didi, Ofir Fargeon, Danielle Arad, Rinat Cohen-Pavon, Gil Sorek, Liron Levin, Dganit Melamed, Liam Aspit, et al.above. Ribosomal RNA was depleted using NEBNext® rRNA Depletion Kit (Bacteria) (New England Biolabs, #E7850). Libraries were constructed using NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina®

(17) Protection against APOE4-associated phenotypes with the longevity-promoting intervention 17α-estradiol in middle-aged male miceCommun Med (Lond)June 10, 2025Cassandra J. McGill, Amy Christensen, Wenjie Qian, Max A. Thorwald, Jose Godoy Lugo, Sara Namvari, Olivia S. White, Caleb E. Finch, Bérénice A. Benayoun, Christian J. Pike500 ng of total RNA was subjected to ribosomal-RNA depletion using the NEBNext rRNA Depletion kit (E7850L; New England Biolabs, Ipswich, MA), according to the manufacturer’s protocol. Strand-specific RNA-seq

(18) Chalkophore mediated respiratory oxidase flexibility controls M. tuberculosis virulenceElifeJune 5, 2025John A Buglino, Yaprak Ozakman, Chad E Hatch, Anna Benjamin, Derek S Tan, Michael S Glickmantotal RNA underwent ribosomal depletion with the NEBNext rRNA Depletion Kit (Bacteria) (NEB catalog # E7850) and library preparation with the TruSeq Stranded Total RNA LT Kit (Illumina catalog # RS-122–1202)

(19) Rifampin mediated decapsulation and enhanced bacterial clearance: a new mechanism of rifamycinsEBioMedicineJune 4, 2025Gi Young Lee, Jeongmin Songremaining rRNA in the sample was further depleted with NEBNext® rRNA Depletion Kit for bacteria (NEB cat# E7850L). After rRNA depletion, a random-primed double-stranded cDNA (dscDNA) library was prepared with the

(20) TEAL-Seq: targeted expression analysis sequencingmSphereMay 27, 2025Georgia Doing, Priya Shanbhag, Isaac Bell, Sara Cassidy, Efthymios Motakis, Elizabeth Aiken, Julia Oh, Mark D. AdamsDirectional Library Prep protocol (NEB #E7760S) was used with NEB’s bacterial rRNA depletion kit (NEB # E7850S) to make bulk RNA-seq libraries for whole transcriptome sequencing. 100 ng RNA input was used, and adaptors

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NEBNext rRNA Depletion Kit (Bacteria)

NEBNext rRNA Depletion Kit (Bacteria) from New England Biolabs

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NEBNext rRNA Depletion Kit (Bacteria) from New England Biolabs

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