NEBGolden GateAssembly Kit (BsmBI-v2) E1602S from New England Biolabs

Product Specs
ItemNEBGolden GateAssembly Kit (BsmBI-v2)
CompanyNew England Biolabs
Price $164.00 Inquire
Catalog NumberE1602S
Quantity20 reactions
ApplicationsDNA Manipulation
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Expression of a phage RecET recombinase system improves detection of shorter DNA fragments by an Acinetobacter baylyi ADP-ISx antibiotic gene biosensor strainMicroPubl BiolApril 21, 2026Nicholas Rhyan, Issac S. Koshy, Ryan Dinh, Anthony J. VanDieren, Dennis M. Mishler, Jeffrey E. BarrickPhusion High-Fidelity DNA Polymerase,New England Biolabs (M0530L) NEBridge Golden Gate Assembly Kit (BsmBI-v2) ,New England Biolabs (E1602L) Spectinomycin Dihydrochloride Pentahydrate (Spt),GoldBio (S-140-5) Kanamycin Monosulfate (Kan) ,GoldBio

(2) Systematic discovery of pro- and anti-HIV host factors in primary human CD4+ T cellsCellApril 20, 2026Ujjwal Rathore, Eli Dugan, Hunter Thornton, Vigneshwari Easwar Kumar, Rama Dajani, Ryan C. Burdick, Janet M. Young, Zachary Steinhart, Reanna Lao, Krista A. Delviks-Frankenberry, et al.(Addgene, cat. no. 96923) using NEB Golden Gate Assembly Kit (BsmBI-v2, New England Biolabs, cat. no. E1602L) following the manufacturer’s protocol. Individual sgRNAs for arrayed CRISPRa validations were constructed

(3) Systematic Evaluation Defines the Limits of Ferroptosis in Cancer TherapybioRxivMarch 17, 2026Kenji M. Fujihara, Azmi Aziz, Behnia Akbari, Maria Gutierrez-Perez, Gregory Francis, Siham Zentout, Kameng Wu, Nicholas J. Clemons, Erdem M. Terzi, Michael E. Pacold, et al.pLentiCRISPRv2 (Cas9 libraries) or pRDA-550 (enAsCas12a libraries) using the NEBridge Golden Gate Assembly Kit (E1602L, New England Biolabs). Assembly reactions were cycled 65 times according to the manufacturer’s protocol.

(4) Mitotic BLM functions are required to maintain genomic stabilityNucleic Acids ResMarch 10, 2026Tamara Eleanore Hamann, Angela Wieland, Farbod Mohseni, Kruno Vukušić, Andrea Tirincsi, Rene Wardenaar, Marialucrezia Losito, Iris Harmsen, Ipek Ilgin Gönenc, Bernd Wollnik, et al.the pENTRY vectors with the acceptor vector pSG3651 using the NEBridge Golden Gate Assembly Kit (NEB #E1602), and error-free assembly was confirmed by DNA sequencing. The DNA insert sequences for the individual

(5) Bacterial Schlafen proteins mediate phage defenceNature MicrobiologyMarch 9, 2026Veronica Perez Taboada, Yimo Wu, Riley Cassidy, Kirill E. Medvedev, Luuk Loeff, Anna Nemudraia, Artem Nemudryisynthesized by Twist Biosciences and assembled using NEBridge Golden Gate Assembly Kit (BsmBI-v2) (NEB, E1602S). For protein expression, the genes encoding for RorSlfn5 and T5.142 were cloned into the pDF0118 (Addgene

(6) Reprogramming mRNA localization by targeted RNA-protein interferencebioRxivMarch 2, 2026Devon E. Mason, Diptankar Bandyopadhyay, Neal Jiwnani, Brigitte Meyer, Stavroula Milicloned into BsmBI sites. gRNA arrays were generated using the NEBridge Golden Gate Assembly kit (NEB; E1602S) using PCR-generated gRNA fragments containing unique overlapping overhangs (Supplementary Table S1). For

(7) Precise control of transcription condensates across S phase balances linker histone expression with DNA replication, ensuring genome stabilityMolecular CellFebruary 19, 2026Carlos Origel Marmolejo, Celina Sanchez, Erin Helms, Melissa J. McEvoy, Juyoung Lee, Marcel Werner, Paige Roberts, Stephan Hamperl, Joshua C. SaldivarDuolink In Situ Detection Reagents Green,Millipore Sigma,Cat#DUO92014 NEB Golden Gate Assembly Kit (BsmBI-v2),New England Biolabs,Cat#E1602S ViewRNA Cell Plus Assay Kit,ThermoFisher Scientific,Cat#88-19000 "Viewrna Cell Plus Probe Set, Alexa

(8) Scalable TCR synthesis and screening enables antigen reactivity mapping in vitiligoImmunityFebruary 10, 2026Stephanie A. Gaglione, Rachit S. Mukkamala, Chirag Krishna, Blake E. Smith, Marc H. Wadsworth, Scott A. Jelinsky, Caleb R. Perez, Laura Schmidt-Hong, Erica L. Katz, Kyle J. Gellatly, et al.KK2601 NEBridge Ligase Master Mix,NEB,Cat#M1100 NEBridge Golden Gate Assembly Kit (BsmBI-v2),NEB,Cat#E1602 NEBridge Golden Gate Assembly Kit (BsaI-HF-v2),NEB,Cat#E1601 BsaI-HF-v2,NEB,Cat#R3733 BsmBI-v2,NEB,Cat#R0739 BbsI-HF,NEB,Cat#R3539 SapI,NEB,Cat#R0569 FastDigest XbaI

(9) A fully synthetic Golden Gate assembly system for engineering a Pseudomonas aeruginosa phiKMV-like phageProceedings of the National Academy of SciencesJanuary 27, 2026Andrew P. Sikkema, Kaitlyn E. Kortright, Hemaa Selvakumar, Jyot Antani, Benjamin K. Chan, Matthew Davidson, Max Hopkins, Benjamin Newman, Vladimir Potapov, Cecilia A. Silva-Valenzuela, et al.fragment. Assembly reactions were set up using the NEBridge Golden Gate Assembly Kit (BsmBI-v2) (NEB, E1602) as follows: 2 µL 10X T4 DNA Ligase Buffer, 2 µL BsmBI-v2 NEBridge Golden Gate Enzyme Mix (a mixture

(10) Construction and Immunogenicity Evaluation of a Recombinant Infectious Bronchitis Virus H120-Based Vaccine in Broiler ChickensAnimals (Basel)January 22, 2026Ali Nayef, Sara Jibreen, Mustafa Ababnehfragment (measured by Qubit 4), and 2 μL of the NEBridge Golden Gate Assembly Kit (BsmBI-v2) (NEB #E1602) (New England Biolabs, Ipswich, MA, USA) in 1× T4 DNA Ligase Reaction Buffer. The reaction mixtures

(11) Assessment of the phi6 lysis system using genetic complementation and heterologous expression in Escherichia coli and Pseudomonas syringaeFrontiers in MicrobiologyJanuary 7, 2026Devyn Del Curto, Micaela Zamora, Greyson Lasley, Rabia Khan, Katlyn Montalbano, J. Bryce Ricken, Jesse CahillIntegrated DNA Technologies (IDT), with flanking BsmBI sites to support Golden Gate assembly (NEB #E1602). Phi6 genomic cDNA segments were produced using IDT gblocks and used with NEB’s BsaI-HF v2 kit (NEB

(12) A pooled Cell Painting CRISPR screening platform enables de novo inference of gene function by self-supervised deep learningNat CommunDecember 16, 2025Srinivasan Sivanandan, Bobby Leitmann, Eric Lubeck, Mohammad Muneeb Sultan, Panagiotis Stanitsas, Navpreet Ranu, Alexis Ewer, Jordan E. Mancuso, Zachary F. Phillips, Albert Kim, et al.(sgRNA library), 2.5 µL T4 DNA Ligase Buffer, 2.5 µL of NEB Golden Gate Enzyme Mix (BsmBI-v2) Mix (NEB, E1602L), and molbio grade water added to a total of 50 µL reaction solution. The PCR strip tube with solution

(13) Rab23 GTPase and IFT43 regulate the trafficking of prostaglandin E receptor 4 to primary ciliaCommunications BiologyDecember 10, 2025Yewei Chen, Yating Zhou, Yao Qi, Tingting Miao, Jianan Shi, Daqing Jin, Tao P. Zhongfrom Dali Li’s lab50. sgRNA expression plasmids were constructed using the Golden Gate cloning method (E1602L, New England Biolabs). Briefly, oligonucleotides for sgRNA were denatured at 95°C for 1 min, followed

(14) Bipolar and schizophrenia risk gene AKAP11 encodes an autophagy receptor coupling the regulation of PKA kinase network homeostasis to synaptic transmissionNature CommunicationsNovember 28, 2025You-Kyung Lee, Cong Xiao, Xiaoting Zhou, Le Wang, Meghan McReynolds, Zhiping Wu, Xian Han, Eric Purisic, Henry Kim, Xianting Li, et al.the Lenti-U6-dcas9-krab-Puro (Addgene #71236) plasmid through the Golden Gate DNA Assembly kit (NEB, E1602S) and confirmed by Sanger sequencing. WT human i3N PSC was transduced with lentiviruses overexpressing

(15) Myogenic enhancer snatching promotes adipogenic differentiation during epigenetic reprogramming mediated by lineage-specific transcription factorsCellular and Molecular Life SciencesNovember 19, 2025Mingyang Hu, Zhixiang Xu, Jiahao Sun, Linmi Li, Pengxuan Liu, Honghong Zhou, Xiaolong Qi, Weike Ren, Yujia Liu, Shuya Chen, et al.construct. Plasmid cloning Lenti-CRISPR-V2 plasmids were linearized using BsmBI restriction endonuclease (NEB, E1602S). The sgRNA sequences, which were listed in Supplementary Table 2, were cloned into the linearized Lenti-CRISPR-V2

(16) A peptide display system identifies a potent mutant β-melanocyte-stimulating hormone agonist of melanocortin-4 receptorCell GenomicsNovember 12, 2025Ann Lin, Kaitlyn Spees, Raeline Valbuena, Jakob Wirbel, Aravind Natarajan, Nora Enright, Ami S. Bhatt, Michael C. Bassik(IDT, Table S3). Plasmids were constructed using Golden Gate Assembly (New England Biolabs, cat no. E1602L) and transformed into One Shot Stbl3 chemically competent e. coli cells (ThermoFisher Scientific, cat

(17) A modular high-throughput approach for advancing synthetic biology in the chloroplast of ChlamydomonasNature PlantsNovember 3, 2025René M. Inckemann, Tanguy Chotel, Michael Burgis, Cedric K. Brinkmann, Laura Andreas, Jessica Baumann, Priyati Sharma, Melanie Klose, James Barrett, Fabian Ries, et al.Golden Gate Assembly Kit from New England Biolabs for both BsaI and BsmBI enzymes (NEB #E1601 and NEB #E1602). The reaction mixtures, prepared without specifying volumes, included a vector at 10 fmol, inserts

(18) Functional and antigenic constraints on the Nipah virus fusion proteinbioRxivOctober 29, 2025Brendan B. Larsen, Sheri Harari, Risako Gen, Cameron Stewart, David Veesler, Jesse D. Bloom(50fmol/μL), 1 μL PCR amplified oPool insert (100fmol/μL), 2 μL 10x T4 DNA Ligase Buffer (NEB, Cat. No. E1602S), 1 μL NEB Golden Gate Enzyme Mix BsmBI-v2 (NEB, Cat. No. E1602S), and 15 μL of water. Reactions were

(19) High-resolution spatial mapping of cell state and lineage dynamics in vivo with PEtracerScienceOctober 16, 2025Luke W. Koblan, Kathryn E. Yost, Pu Zheng, William N. Colgan, Matthew G. Jones, Dian Yang, Arhan Kumar, Jaspreet Sandhu, Alexandra Schnell, Dawei Sun, et al.pre-digested backbone diluted to 25 ng/μL), 2 μL of NEBridge Golden Gate Assembly Kit (BsmBI-v2) (NEB, E1602L), 2 μL T4 ligase buffer, and 7 μL of nuclease free water. Assemblies were carried out following manufacturer’s

(20) Heterologous plastoquinone production using a newly identified O 2 ‐dependent cyanobacterial hydroxylaseThe FEBS JournalOctober 2, 2025Morgane Roger‐Margueritat, Margot Beltran, Juliette Schnoebelen, Laura Flandrin, Wafa Rezali, Eline Michel, Sophie S. Abby, Fabien Pierrelone PlqH TU in the pTU‐2a‐RFP plasmid (#72958) using NEBridge Golden Gate Assembly Kit (BsmBI‐v2, #E1602, New England BioLabs) in accordance with the manufacturer's protocol. The assembly of DNA fragments

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NEBGolden GateAssembly Kit (BsmBI-v2)

NEBGolden GateAssembly Kit (BsmBI-v2) from New England Biolabs

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NEBGolden GateAssembly Kit (BsmBI-v2) from New England Biolabs

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