Human IFN-gamma Quantikine ELISA Kit DIF50C from R&D Systems, a Bio-Techne Brand

Product Specs
ItemHuman IFN-gamma Quantikine ELISA Kit
CompanyR&D Systems, a Bio-Techne Brand
Price $680.00Supplier Page View Company Product Page
Catalog NumberDIF50C
Quantity1 Kit (also 1 Pack (50 Plates), 1 Pack (6 Plates))
ApplicationsELISA
Detection Range15.60 - 1000 pg/mL
ReactivityHuman
Sample TypeCell Culture Supernates (100 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL)
Sensitivity5.69 pg/mL
Detection TargetIFN-gamma
Assay Time4.5 hours
StorageStore the unopened product at 2 - 8 °C. Do not use past expiration date
NCBI Full Gene Nameinterferon gamma
NCBI Gene AliasesIFG, IFI, IMD69

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) AAV immuno-gene therapy platform delivering vectorized cytokines defines a new modality for high-grade glioma treatmentMolecular Therapy OncologyJune 18, 2026Akela Kuwahara, Ethan M. Fenton, Chris Lovejoy, Tian Lee, Lin Wang, Miko Fogarty, Jangham Jung, Syuan-Ming Guo, Lucy H. Maynard, Andrew P. May, et al.instructions (human IFNα [PBL Cat# 41135-1], human IFNβ [PBL Cat#41410], and human IFNγ [R&D Systems Cat#: DIF50C]). Experiments were performed in technical quadruplicate. For potency described in Figure 6A, HEK293T

(2) A machine learning model integrating gut microbiota biomarkers for predicting neurological recovery after cerebral hypoxia-ischemia: a single-center study augmented with public dataFrontiers in PharmacologyMarch 31, 2026Ling Kong, Chunhua WangTNF-α ELISA Kit, Cat. No. DTA00D; Human IL-6 ELISA Kit, Cat. No. D6050; Human IFN-γ ELISA Kit, Cat. No. DIF50C; R&D Systems, Minneapolis, MN, United States), following the manufacturer’s protocols. Absorbance was

(3) Blinatumomab-driven T-cell activation in αβ and γδ T-cell subsets: insights from in vitro assaysFrontiers in ImmunologyFebruary 6, 2026Miriam Kelm, Nourhan Nasr, Sonja Bendig, Dieter Kabelitz, Marta Lustig, Heiko Trautmann, Anna Laqua, Christian Peters, Daniela Wesch, Hans-Heinrich Oberg, et al.2. Enzyme-linked immunosorbent assay Cell culture supernatants were analyzed for Interferon-γ (IFNγ; DIF50C), Granzyme B (GrzB; DGZB00), Perforin (QK8011), Granulysin (Gnly; DY3138), Perforin (Prf; QK8011) and

(4) SLC6A14-mediated glutamine promotes SYTL4–CXCL8 axis activation to drive gemcitabine resistance and immune evasion in pancreatic cancerExperimental & Molecular MedicineDecember 25, 2025Hyeon Woong Kang, Ju Hyun Kim, Jae Woong Jeong, Sungsoon Fang, Won-Gun Yun, Hye-Sol Jung, Wooil Kwon, Jin-Young Jang, Hyo Jung Kim, Joon Seong ParkIFN-γ-specific antibodies. IFN-γ levels were measured using Quantikine ELISA kits (R&D Systems, cat. no. DIF50C) according to the manufacturer’s protocol. Following supernatant removal, cells were washed with PBS,

(5) NovumRNA: Accurate prediction of non-canonical tumor antigens from RNA sequencing dataiScienceOctober 17, 2025Markus Ausserhofer, Dietmar Rieder, Manuel Facciolla, Raphael Gronauer, Giorgia Lamberti, Rebecca Lisandrelli, Serena Pellegatta, Zlatko Trajanoski, Francesca Finotello,Thermo Fisher,Cat#15596026 RNeasy Mini Kit,Qiagen,Cat#74104 RNase-Free DNase Set,Qiagen,Cat#79254 IFN-γ ELISA,R&D Systems,Cat#DIF50C ,, Deposited data,Deposited data,Deposited data ,, NovumRNA source code,This paper,https://github.com/ComputationalBiomedicineGroup/NovumRNA NovumRNA

(6) Geometric Tuning of Cytokine Receptor Association Modulates Synthetic Agonist SignalingbioRxivOctober 17, 2025Marc Expòsit, Mohamad Abedi, Aditya Krishnakumar, Shruti Jain, Ta-Yi Yu, Timothy R. Hercus, Divij Mathew, Sophie Gray-Gaillard, Zhijie Chen, William S. Grubbe, et al.were measured using a commercially available ELISA kit (R&D Quantikine Human IFNy kit, Biotechne Cat# DIF50C)33. RNA-seq and GO term annotation CD4+ T cells were isolated and stimulated overnight with IL-7 or

(7) Reduced ALDH1A1 expression in multiple myeloma cells increases resistance to daratumumab via downregulation of retinoic acidCell Mol Life SciOctober 7, 2025Sujie Zheng, Jing He, Xiaolu Song, Ye Peng, Wufeng Yuan, Xiangmin TongINF-gamma measurement Commercial enzyme-linked immunoabsorbent assay (ELISA) kits (NBP3-11726, DGZB00, and DIF50C, Novus Biologicals) were used measuring above granule contents, following protocols reported previously

(8) Blocking NRF2 Translation by Inhibition of Cap-Dependent Initiation Sensitizes Lymphoma Cells to Ferroptosis and CAR T-cell ImmunotherapyCancer researchOctober 1, 2025Manara P, Newsam AD, Venkatarame Gowda Saralamma V, Cunningham TA, Lazenby D, Ho JJD, Russo MV, Youssfi Alaoui A, Chahar D, Bilbao Martinez A, et al.replicates per biological replicate. ELISA kits specific for mouse TNFα and IFNγ (R&D Systems, #DTA00D and #DIF50C) were used according to manufacturer’s protocol to quantify cytokine levels. In Vivo GCB PDX Synergy

(9) Endoglin-Directed CAR T Cells Comprehensively Target Tumors in Advanced SarcomasCancer Immunology ResearchOctober 1, 2025Harrison R. Berger, Malina Maharana, Jeneffer Mirabal, Lyazat Kurenbekova, Alberto Delaidelli, Atreyi Dasgupta, Ahmed Z. Gad, Mohamed F. Sheha, Sybrina S. Kerr, Ada I. Ozcan, et al.The supernatant was analyzed using Quantikine ELISA kits (R&D systems) for IL-2 (#D2050) and IFN-γ (#DIF50C) according to the manufacturer’s instructions. In another experiment, the MIILIPLEX MAP Human CD8+ T-cell

(10) TAGAP restrains myeloid and T cell activation in inflammatory bowel diseaseFrontiers in ImmunologySeptember 22, 2025Rui-Ci Lin, Zhifei Shao#K15049D-1), Human IL-2 ELISA kit (Invitrogen; BMS2212), and Humun IFN-γ quantikine ELISA kit (R&D Systems; #DIF50C) were used to measure the cytokine/chemokine levels from the supernatant of cultured human macrophages

(11) Baricitinib and Infliximab Mitigate the Endothelial-to-Mesenchymal Transition (EndMT) Induced by Cytokines in HUVECsInternational journal of molecular sciencesSeptember 5, 2025Barilli A, Visigalli R, Recchia Luciani G, Crescini E, Dall'Asta V, Rotoli BM.

(12) Immune targeting of triple-negative breast cancer through a clinically actionable STING agonist-CAR T cell platformCell Reports MedicineJuly 15, 2025Yuqing Zhang, Zehua Li, Jessica Ritter, Elliott J. Brea, Navin R. Mahadevan, Deborah A. Dillon, Sung-Rye Park, Eric Minwei Liu, Michael Y. Tolstorukov, William E. McGourty, et al.,R&D Systems,Cat# D8000C Human IFNβ ELISA kit,R&D Systems,Cat# DIFNB0 Human IFNγ ELISA kit,R&D Systems,Cat# DIF50C Human Granzyme B ELISA kit,R&D Systems,Cat# DGZB00 Human TNFα ELISA kit,R&D Systems,Cat# DTA00D CellTiter-Glo Luminescent cell viability assay

(13) Identification of matrix stiffness-related molecular subtypes in HCC via integrating multi-omics analysis and machine learning algorithmsJournal of translational medicineJuly 1, 2025Li H, Zhang J, Shi Y, Wang H, Yang R, Wu S, Li Y, Yang X, Liu Q, Sun L.centrifuged (14,000 rpm) and analyzed for IFN-γ and IL-2 secretion using commercial ELISA kits (R&D Systems: #DIF50C for IFN-γ, #QK202 for IL-2) following standard protocols. HepG2-HSCs coculture model A HepG2-HSCs

(14) Human cancer-targeted immunity via transgenic hematopoietic stem cell progenyNat CommunJuly 1, 2025Theodore S. Nowicki, Nataly Naser Al Deen, Cole W. Peters, Begoña Comin-Anduix, Egmidio Medina, Cristina Puig-Saus, Ignacio Baselga Carretero, Paula Kaplan-Lefko, Mignonette H. Macabali, Ivan Perez Garcilazo, et al.media plated in a total of 100 μL of R10 (+Il2), then incubated for 20 h. IFNy ELISA (R&D Systems, cat#DIF50C) was performed per manufacturer instructions as above. Briefly, co-culture supernatant was used in a

(15) Elevated serum levels of interleukin-18 discriminate Still's disease from other autoinflammatory conditions: results from the European ImmunAID cohortRMD OpenMay 7, 2025Charlotte Girard-Guyonvarc'h, Emiliana Rodriguez, Yvonne M Mueller, Assunta Caruso, Peter D Katsikis, Cem Gabay, The ImmunAID consortiumR&D system (catalogue number DY280) undiluted, and IFN-γ Quantikine ELISA from R&D system (catalogue number DIF50C), undiluted) according to manufacturer’s instructions. When necessary, additional dosages were performed

(16) PAQR5 drives the malignant progression and shapes the immunosuppressive microenvironment of hepatocellular carcinoma by activating the NF-κB signalingBiomark ResMay 7, 2025Ruida Yang, Huanhuan Wang, Cong Wu, Yu Shi, Hanqi Li, Xinyue Bao, Yuqian Yang, Shaoshan Han, Xue Yang, Jie Tao, et al.by ELISA assay. The ELISA kits for detection of IFN-γ and IL-2 were purchased from R&D Systems (#: DIF50C for IFN-γ, #: QK202 for IL-2). qRT-PCR After finishing the designed intervention or co-culture, the

(17) Pan-Variant SARS-CoV-2 Vaccines Induce Protective Immunity by Targeting Conserved EpitopesAdvanced science (Weinheim, Baden-Wurttemberg, Germany)April 1, 2025Shah M, Moon SU, Shin JY, Choi JH, Kim D, Woo HG.to quantify IFN‐γ levels using the Human IFN‐gamma Quantikine ELISA Kit (R&D Systems, catalog number DIF50C, MN, USA). Although CD3 and CD28 activation induced a high background level of T‐cell activation, the

(18) Sodium citrate pretreatment enhances CAR-T cell persistence and anti-tumor efficacy through inhibition of calcium signalingFront ImmunolMarch 17, 2025Xuechen Yin, Wenwen Chen, Xudong Ao, Luxia Xu, Jiujiu Cao, Tinghui Huang, Junqing Liang, Jianhua Hu, Jiaqi Liu, Xinping Wang, et al.supernatants were collected for ELISA analysis. ELISA was performed using an ELISA kit (R&D Systems, DIF50C, DTA00D) following the manufacturer’s instructions. 2.6 Cytotoxicity detection The cytotoxicity of

(19) GMP manufacturing of umbilical cord blood mesenchymal stem cell-released exosomes and verification of wound healing efficacyNanotheranosticsMarch 3, 2025Jae-Yong Lee, Jaehyoung Kim, Kyuheum Na, Hee-Jin Ahn(ELISA) The levels of each cytokine, including TNF-α (Cat. no. DTA00D, R&D System, NE, USA), IFN-γ (Cat. No. DIF50C, R&D System, NE, USA), IL-1β (Cat. no. DLB50, R&D System, NE, USA) were detected and quantified by immunoassay

(20) Mapping variant effects on anti-tumor hallmarks of primary human T cells with base-editing screensNature BiotechnologyMarch 1, 2025Zachary H. Walsh, Parin Shah, Neeharika Kothapalli, Shivem B. Shah, Gergo Nikolenyi, D. Zack Brodtman, Giuseppe Leuzzi, Meri Rogava, Michael Mu, Patricia Ho, et al.were measured with the appropriate Quantikine ELISA kit (R&D Systems, TNF, DTA00D; IL-2, D2050; IFN-γ, DIF50C) following the manufacturer’s instructions. The following supernatant dilutions were used for input

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Human IFN-gamma Quantikine ELISA Kit

IFN-gamma ELISA

IFN-gamma ELISA

IFN-gamma ELISA

Human IFN-gamma Quantikine ELISA Kit from R&D Systems, a Bio-Techne Brand

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R&D Systems, a Bio-Techne Brand

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Human IFN-gamma Quantikine ELISA Kit from R&D Systems, a Bio-Techne Brand

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