Description
CST's PathScan® Acetyl-Histone H4 (Lys12) Sandwich ELISA Kit #7228 detects endogenous levels of histone H4 when acetylated at Lys12. As shown in Figure 1 using the Acetyl-Histone H4 (Lys12) Sandwich ELISA Kit #7228, a high level of acetylation at Lys12 on histone H4 is detected in NIH/3T3 cells when treated with TSA. The level of total histone H4 (acetylated and non-acetylated) remains unchanged as shown by Western analysis (Figure 1). Similar results are obtained when COS and Jurkat cells are treated with TSA (data not shown). This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species