Description
Intended Uses: For the quantitative detection of Human Lyso-platelet activating factor (lyso-PAF) concentration in serum, plasma and other biological fluids.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate lyso-PAF in Human serum, plasma. An antibody specific for lyso-PAF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any lyso-PAF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for lyso-PAF is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of lyso-PAF bound in the initial step. The color development is stopped and the intensity of the color is measured