Description
Principle of the Assay: The Picokine Rat Fgf21 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Rat Fgf21 with a 96-well strip plate that is pre-coated with antibody specific for Fgf21. The detection antibody is a biotinylated antibody specific for Fgf21. The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated polyclonal antibody from goat. The kit contains recombinant Rat Fgf21 with immunogen: Expression system for standard: E.coli, Immunogen sequence: Y30-S208. The kit is analytically validated with ready to use reagents. To measure Rat Fgf21, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly propotional to Rat Fgf21 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Rat Fgf21 in the sample: