Description
Description: This assay employs a two-site sandwich ELISA to quantitate CD46 in samples. An antibody specific for CD46 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CD46 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CD46 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CD46 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Overview: CD5 is a cluster of differentiation found on a subset of IgM-secreting B cells called B-1 cells, and also on T cells. B-1 cells have limited diversity of their B-cell receptor due to their lack of the enzyme terminal deoxynucleotidyl transferase (TdT) and are potentially self-reactive. CD5 serves to mitigate activating signals from the BCR so that the B-1 cells can only be activated by a very strong stimuli (such as bacterial proteins) and not by normal tissue proteins. CD5 was used as a T-cell marker until monoclonal antibodies against CD3 were developed. In humans, the gene is located on the long arm of chromosome 11. There is no confimed ligand for CD5. T cells express higher levels of CD5 than B cells. CD5 is upregulated on T cells upon strong activation