Description
Principle of the Assay: The Picokine Human NT5E Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human NT5E with a 96-well strip plate that is pre-coated with antibody specific for NT5E. The detection antibody is a biotinylated antibody specific for NT5E. The capture antibody is polyclonal antibody from sheep, the detection antibody is polyclonal antibody from sheep. The kit contains recombinant Human NT5E with immunogen: Expression system for standard: CHO; Immunogen sequence: W27-K547. The kit is analytically validated with ready to use reagents. To measure Human NT5E, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly propotional to Human NT5E in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human NT5E in the sample: