Description
Intended Uses: Enzyme Immunoassay for the determination of C-Reactive Protein in human serum and plasma. For research use only. not for use in diagnostic procedures.
Principle of the Assay: Microtiterstrips coated with anti-CRP antibody are incubated with diluted standard sera and samples. During this incubation step CRP is bound specifically to the wells. After removal of the unbound serum proteins by a washing procedure, the antigen-antibody complex in each well is detected with specific peroxidase-conjugated antibodies. After removal of the unbound conjugate, the strips are incubated with a chromogen solution containing tetramethylbenzidine and hydrogen peroxide: a blue colour develops in proportion to the amount of immunocomplex bound to the wells of the strips. The enzymatic reaction is stopped by the addition of 0.5M H2S04 and the absorbance values at 450 nm are determined. A standard curve is obtained by plotting the absorbance values versus the corresponding standard values. The concentration of CRP in samples is determined by interpolation from the standard curve