Description
Intended Uses: This 6KPGF1a ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of 6KPGF1a in biological samples. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Principle of the Assay: 6KPGF1a ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-6KPGF1a antibody and an 6KPGF1a-HRP conjugate. The assay sample and buffer are incubated together with 6KPGF1a-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the 6KPGF1a concentration since 6KPGF1a from samples and 6KPGF1a-HRP conjugate compete for the anti-6KPGF1a antibody binding site. Since the number of sites is limited, as more sites are occupied by 6KPGF1a from the sample, fewer sites are left to bind 6KPGF1a-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The 6KPGF1a concentration in each sample is interpolated from this standard curve