Description
Description: The FLICA 660 Caspase-3/7 kit uses a target sequence (DEVD) sandwiched between a far-red fluorescent 660 dye and a fluoromethylketone (FMK) to make a quick and flexible method to analyze active caspase-3/7 in apoptotic cells.
Priciple of the assay: Caspase FLICA kits measure apoptosis by detecting active caspases in whole, living cells. These kits do not work by using antibodies or as an ELISA. Instead, their methodology is based on a unique cell-permeable and non-cytotoxic reagent called the Fluorochrome Inhibitor of Caspases (FLICA). The FLICA reagent contains a caspase inhibitor sequence linked to a far-red fluorescent 660 dye.
The Caspase FLICA Kits are suitable for cells in suspension, adherent cells, thin tissue sections (but not fixed or paraffin-embedded cells) from many species including mammalian, insect and yeast. Different cell types, e.g. HeLa, primary neurons, macrophages and lymphocytes have also been successfully studied with these kits. This kit can be used with either a flow cytometer or fluorescence microscope