Description
Intended Uses: This sandwich kit is for the accurate quantitative detection of Human Interleukin 18 Receptor Accessory Protein (also known as IL-18RAP) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.
Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human IL-18RAP antibody. IL-18RAP present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human IL-18RAP Antibody is added and binds to IL-18RAP in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated IL-18RAP antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human IL-18RAP. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm