Description
Principle of the Assay: Unlike most cell-based BrdU assay kits that can only measure qualitative differences in BrdU incorporation, the CytoSelect BrdU Competitive ELISA kit allows quantitative measurement of BrdU. The unknown BrdU-containing samples or free BrdU standards are first added to a BrdU conjugate preadsorbed microplate. After a brief incubation, an anti-BrdU monoclonal antibody is added, followed by an HRP conjugated secondary antibody. The BrdU content in unknown samples is determined by comparison with a predetermined BrdU standard curve: