Description
Principle: This human CD93 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CD93 has been precoated onto 96-well plates. Standards(NSO, A24-K580) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD93 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CD93 amount of sample captured in plate.
Description: The Fast version of Picokine ELISA kits, assay takes less than 1.5 hours. Detect Human C1Q R1/CD93 with <10pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, serum, plasma(heparin, EDTA), saliva and urine. This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. C1Q R1/CD93 tissue specificity: Highly expressed in endothelial cells, platelets, cells of myeloid origin, such as monocytes and neutrophils. Not expressed in cells of lymphoid origin.