Description
Principle of the Assay: Reduced glutathione (GSH) is a tripeptide which composed of glutamic acid, glycine and cysteine. It is a kind of low molecular scavenger, which can remove O2-, H2O2, and LOOH. Beside as the main thiol compound of non-protein in the organization, GSH is the substrate of GSH-PX and GST which is indispensable to decomposing hydrogen peroxide for the two enzyme. What's more, it can stabilize the enzyme containing thiol and prevent hemoglobin and other auxiliary factors from the oxidative damage. Recently, it is proved that GSH is also involved in the recovery of vitamin E to the reduction state. When lacking or depletion of GSH, it may cause producing toxic effects or increasing the toxic effects of many chemicals or environmental factors. It may be related to the increase of oxidative damage, so the amount of GSH is a vital factor to measure the body's antioxidant ability.Dithio-dinitrobenzoic acid can react with sulfhydryl compound to generate a yellow compound. Measure the OD value and calculate the GSH content indirectly