Description
Intended Uses: This sandwich kit is for the accurate quantitative detection of Rat Granulocyte Colony Stimulating Factor (also known as G-CSF) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.
Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Rat G-CSF antibody. G-CSF present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat G-CSF Antibody is added and binds to G-CSF in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated G-CSF antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat G-CSF. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm