Description
Principle of the Assay: Lactate Assay Kit measures lactate within biological samples. Lactate is oxidized by lactate oxidase into pyruvate and hydrogen peroxide. The hydrogen peroxide is then detected with a highly specific Fluorometric probe. Horseradish peroxidase catalyzes the reaction between the probe and hydrogen peroxide, which bind in a 1:1 ratio. Samples and standards are incubated for 30-45 minutes and then read with a standard 96-well Fluorometric plate reader (Figure 1). Samples are compared to a known concentration of lactate standard within the 96-well microtiter plate format.