Description
Principle of the Assay: NADP+ /NADPH Assay Kit is a convenient quantitative tool that measures NADP+ and NADPH within biological samples. The assay is based on an enzymatic cycling reaction in which NADP+ is reduced to NADPH. NADPH reacts with a fluorometric probe that produces a product which can be measured with a standard 96-well fluorometric plate reader. The intensity of the product fluorescence is proportional to the NADP+ and NADPH within a sample. A simple acid or base treatment will differentiate NADPH from NADP+ within a sample. Samples and standards are incubated for 1-2 hours and then read at lambdaex = 530-570 nm/ lambdaem = 590-600 nm (Figure 1). Samples are compared to a known concentration of NADP+ standard within the 96-well microtiter plate format.