Description
Intended Uses: This sandwich kit is for the accurate quantitative detection of Rat Keratin 15 (also known as KRT15) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Rat KRT15 antibody. KRT15 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat KRT15 Antibody is added and binds to KRT15 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated KRT15 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat KRT15. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm