Description
Intended Uses: This sandwich kit is for the accurate quantitative detection of Fish Mannma Binding Protein/Mannan Binding Lectin (also known as MBP/MBL) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.
Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Fish MBP/MBL antibody. MBP/MBL present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Fish MBP/MBL Antibody is added and binds to MBP/MBL in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated MBP/MBL antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Fish MBP/MBL. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm