Description
Principle of the Assay: This kit uses Indirect-Competitive-ELISA as the method. It can be used to detect Fluoroquinolones (QNs) in samples, such as honey, animal tissues (chicken, porcine, fish, and shrimp) milk, eggs, etc. This kit is composed of Micro ELISA Plate, HRP conjugate, antibody, standard and other supplementary reagents. The micro-plate provided in this kit has been pre-coated with QNs antigen. During the reaction, QNs in the samples or standard competes with QNs antigen coated on the solid phase supporter for QNs antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each micro plate well, and TMB substrate is added for color development. There is a negative correlation between the OD value of samples and the concentration of QNs. The concentration of QNs in the samples can be calculated by comparing the OD of the samples to the standard curve