Description
Intended Uses: This sandwich kit is for the accurate quantitative detection of Porcine Signal Transducer and Activator of Transcription 6 (also known as STAT6) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Porcine STAT6 antibody. STAT6 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Porcine STAT6 Antibody is added and binds to STAT6 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated STAT6 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Porcine STAT6. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm