Description
Intended Uses: This sandwich kit is for the accurate quantitative detection of Bovine Pregnancy Associated Plasma Protein A (also known as PAPP-A) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Bovine PAPP-A antibody. PAPP-A present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Bovine PAPP-A Antibody is added and binds to PAPP-A in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated PAPP-A antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Bovine PAPP-A. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm