Description
Intended Use: The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration ofCHOLESTEROL 8 beta HYDROXYLASE in the sample, This CHOLESTEROL 8 beta HYDROXYLASE ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versusCHOLESTEROL 8 beta HYDROXYLASE concentration. The concentration ofCHOLESTEROL 8 beta HYDROXYLASE in the samples is then determined by comparing the O.D. of the samples to the standard curve