Description
Principle of the Assay: This kit uses Indirect-Competitive-ELISA as the method. It can detect Tetracyclines (TCs) in samples, such as tissues, honey, eggs, urine and other samples. This kit is composed of Micro ELISA Plate pre-coated with coupled antigen, HRP conjugate, antibody, standard and other supplementary reagents. During the detection, after adding standard or sample solution, TCs in the samples competes with pre-coated coupled antigen on the Micro ELISA Plate for TCs antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each micro plate well, and TMB substrate is added for color development. There is a negative correlation between the OD value of samples and the concentration of TCs. The residual quantity of TCs in the samples can be calculated by comparing the OD of the samples to the standard curve