Description
Intended Uses: This sandwich kit is for the accurate quantitative detection of Mouse Coagulation Factor VII (also known as F7) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.
Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Mouse F7 antibody. F7 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse F7 Antibody is added and binds to F7 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated F7 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse F7. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm