Description
Intended Uses: This sandwich kit is for the accurate quantitative detection of Fish Alkaline Phosphatase, Tissue-nonspecific Isozyme (also known as ALPL) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Fish ALPL antibody. ALPL present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Fish ALPL Antibody is added and binds to ALPL in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated ALPL antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Fish ALPL. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm