Description
Intended Uses: This sandwich kit is for the accurate quantitative detection of Rabbit Nitric Oxide Synthase 3, Endothelial (also known as NOS3/ENOS) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Rabbit NOS3/ENOS antibody. NOS3/ENOS present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rabbit NOS3/ENOS Antibody is added and binds to NOS3/ENOS in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated NOS3/ENOS antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rabbit NOS3/ENOS. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm