Description
Test Principle: This assay employs a two-site sandwich ELISA to quantitate MTX1 in samples. An antibody specific for MTX1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MTX1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MTX1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MTX1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Overview: Metaxin 1, is a protein located on the outer membrane of mitochondria, is a component of the mitochondrial protein translocation apparatus.that spans the 6-kb interval separating the glucocerebrosidase gene (GBA) from the thrombospondin III geneon chromosome 3. Metaxin (so named from a Greek word for 'in between') and GBA are transcribed convergently; their major polyadenylation sites are only 431 bp apart. On the other hand, metaxin and the Tsp3 gene are transcribed divergently and share a common promoter sequence. The cDNA from metaxin encodes a 317-amino acid protein which lacks both a signal sequence and a consensus sequence for N-linked glycosylation. Metaxin protein is expressed ubiquitously in tissues of the young adult mouse. No close homologs were found in the DNA or protein databases