Fig 1: Effect of UVA irradiation on growth factors release in SZ95 sebocytes(A) Experimental scheme of UVA irradiation of SZ95 sebocytes.(B) Phase-contrast analysis of SZ95 sebocytes after 4-UVA 5 J/cm2 irradiations.(C) The mRNA expression levels of POMC, EDN1, SCF, and b-FGF in SZ95 sebocytes after 24 and 48h post 1-UVA and 48h post three or 4-UVA 5 J/cm2 irradiations. Results are presented as the mean ± SD of three independent experiments and are expressed as the fold change respect to untreated control cells (*p < 0.05, **p < 0.01).(D) Protein quantitation by ELISA of a-MSH, EDN1, SCF, and b-FGF in SZ95 sebocytes after 24 and 48h post 1-UVA and 48h post three or 4-UVA 5 J/cm2 irradiations. Results are presented as the mean ± SD of three independent experiments and are expressed in absolute quantities (*p < 0.05, **p < 0.01 vs Ctr).(E) The mRNA expression levels and protein quantitation by ELISA of POMC/a-MSH, EDN1, SCF, and b-FGF in SZ95 sebocytes after 72h or 1 week post 7-UVA 5 J/cm2 irradiations. Data represent the mean ± SD of three independent experiments. For mRNA levels, results are expressed as the fold change respect to untreated control cells (**p < 0.01). For ELISA assay, results are expressed in the absolute quantities (**p < 0.01 vs Ctr).
Fig 2: Effect of UVA irradiation on p38MAP kinase and p53 signaling in SZ95 sebocytes(A) Western blot analysis of phospho-p38 protein expression in SZ95 sebocytes after 1-2-4h post irradiation with UVA 2-5-8 J/cm2 ß-tubulin was used as an equal loading control. Representative blots are shown. Densitometric scanning of band intensities was performed to quantify the change of protein expression (control value taken as 1-fold in each case).(B) Western blot analysis of p53 and p21 protein expression in SZ95 sebocytes after 24 and 48h post 1-UVA and 48h post three or 4-UVA 2-5-8 J/cm2. GAPDH was used as an equal loading control. Representative blots are shown. Densitometric scanning of band intensities was performed to quantify the change of protein expression (control value taken as 1-fold in each case).(C) The mRNA expression levels of POMC, EDN1, SCF, and b-FGF in SZ95 sebocytes after 48h of treatment with PFTa 5 µM and one or 3-UVA 5 J/cm2 irradiations. Results are presented as the mean ± SD of three independent experiments and are expressed as the fold change respect to untreated control cells (*p < 0.05, **p < 0.01 vs Ctr; $p < 0.05 vs 1-UVA irradiated cells; §p < 0.05 vs 3-UVA irradiated cells).(D) Protein quantitation by ELISA of aMSH, EDN1, SCF, and b-FGF in SZ95 sebocytes after 48h of treatment with PFTa 5 µM and one or 3-UVA 5 J/cm2 irradiations. Results are presented as the mean ± SD of three independent experiments and are expressed in absolute quantities (**p < 0.01 vs Ctr; $p < 0.05 vs 1-UVA irradiated cells; §p < 0.05 vs 3-UVA irradiated cells).
Supplier Page from Wuhan Fine Biotech Co., Ltd. for Human AMSH(alpha-MSH) ELISA Kit