NEB PCR Cloning Kit (Without Competent Cells) E1203S from New England Biolabs

Product Specs
ItemNEB PCR Cloning Kit (Without Competent Cells)
CompanyNew England Biolabs
Price $179.00 Inquire
Catalog NumberE1203S
Quantity20 reactions
ApplicationsDNA Manipulation
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) The CHI3L1-neutrophil axis drives immune suppression and breast cancer metastatic disseminationJCI InsightMarch 23, 2026Tarek Taifour, Adéline Massé, Yu Gu, Virginie Sanguin-Gendreau, Dongmei Zuo, Bin Xiao, Emilie Solymoss, Yunyun Shen, Hailey Proud, Sherif Samer Attalla, et al.plasmid was generated by cloning of the different elements into a pMiniT plasmid (New England Biolabs E1203S). The plasmid was diluted out in TE Low EDTA buffer (Thermo Fisher Scientific J75793.AP) at a final

(2) Cappable-Seq reveals the transcriptional landscape of stress responses in the bacterial endosymbiont WolbachiaMicrob GenomOctober 27, 2025Youseuf Suliman, Zhiru Li, Amit Sinha, Philip D. Dyer, Catherine S. Hartley, Laurence Ettwiller, Alistair C. Darby, Clotilde K. Carlow, Benjamin L. Makepeacecloned into a single-use stable competent E. coli using the NEB PCR cloning kit (NEB product number E1203S) according to the manufacturer’s instructions. Orthologous gene comparisons To define orthologous

(3) CRISPR-Cas9 screening reveals G2E3 as a novel ubiquitin-linked factor controlling autophagosome-lysosome fusion and cancer cell progressionCell Death DiscovOctober 9, 2025Yumei Gong, Marc Leon, Huaqing Mo, Premkamol Pengpaeng, Hai Yang, Yanxi Lu, Zhiqiang Yin, Alan Benard, Yong Zhou, Robert Grützmann, et al.amplified PCR products were cloned into pMiniT 2.0 using the NEB PCR Cloning Kit (New England Biolabs, Cat# E1203S), and sequencing was performed by Eurofins Genomics. The PCR primers used in this study were designed

(4) PAM-flexible adenine base editing rescues hearing loss in a humanized MPZL2 mouse model harboring an East Asian founder mutationNat CommunAugust 5, 2025Shao Wei Hu, Sohyang Jeong, Luoying Jiang, Hansol Koo, Zijing Wang, Won Hoon Choi, Biyun Zhu, Heeyoung Seok, Yi Zhou, Min Gu Kim, et al.by treatment with T7 endonuclease 1 (M0302S, NEB, Ipswich, MA, USA). For hMPZL2WT mice, PCR cloning (E1203S, NEB) was additionally performed for allele separation, and Sanger sequencing was performed for sequence

(5) DNA methylation at retrotransposons protects the germline by preventing NRF1-mediated activationEMBO ReportsAugust 4, 2025Jessica Leismann, Styliani-Eirini Kanta, Ishita Amar, Anna Szczepińska, Monika Mielnicka, Giuseppe Petrosino, Anke Busch, Marion Scheibe, Pengxiang Wang, Yuan Wang, et al.conversion (EZ DNA Methylation Quick kit, D5005), amplification, cloning into pMiniT™ 2.0 Vectors (NEB, E1203S), followed by transformation and Sanger sequencing of ten clones per probe (Fig. EV3C,D). DNA pulldown

(6) CRISPR/Cas9 Screening Highlights PFKFB3 Gene as a Major Contributor to 5-Fluorouracil Resistance in Esophageal CancerCancers (Basel)May 12, 2025Feng Xue, Hai Yang, Pengyan Xu, Shuman Zhang, Nathalie Britzen-Laurent, Li-Li Bao, Robert Grützmann, Christian Krautz, Christian Pilarskyand transformed into a pMiniT 2.0 vector using an NEB PCR Cloning Kit (New England Biolabs, Germany, #E1203S). The plasmid DNA was isolated from single colony cultures using a GeneJET Plasmid miniprep Kit (Thermo

(7) Exploring the importance of predicted camel NRAP exon 4 for environmental adaptation using a mouse modelAnimal GeneticsFebruary 1, 2025Sung‐Yeon Lee, Bo‐Young Lee, Byeonghwi Lim, Rasel Uzzaman, Goo Jang, Kwan‐Suk Kimwere separated on a 1% agarose gel. Heterozygous alleles were further confirmed through PCR cloning (E1203S, NEB) followed by Sanger sequencing (Macrogen). The sequencing results were analyzed using NCBI's blast

(8) Roflumilast inhibits tumor growth and migration in STK11/LKB1 deficient pancreatic cancerCell Death DiscovMarch 9, 2024Shuman Zhang, Duo Yun, Hao Yang, Markus Eckstein, Gihan Daw Elbait, Yaxing Zhou, Yanxi Lu, Hai Yang, Jinping Zhang, Isabella Dörflein, et al.Supplementary Table 4. Amplicons were subcloned into a plasmid using the NEB® PCR Cloning Kit (NEB, #E1203S) for transformation and several individual colonies were selected and sequenced to determine the clonal

(9) Common as well as unique methylation-sensitive DNA regulatory elements in three mammalian SLC9C1 genesGeneJanuary 30, 2024Cameron C. Gardner, Jason A. Abele, Thomas J. Winkler, Caroline N. Reckers, Sydney A. Anas, Paul F. Jameswere cloned into the pMiniT 2.0 vector using the NEB PCR Cloning Kit (New England BioLabs; Catalog #E1203S) and then transformed into NEB 10-beta competent cells (New England BioLabs; Catalog # C3019H). Plasmid

(10) dCas9/CRISPR-based methylation of O-6-methylguanine-DNA methyltransferase enhances chemosensitivity to temozolomide in malignant gliomaJournal of neuro-oncologyJanuary 1, 2024Zapanta Rinonos S, Li T, Pianka ST, Prins TJ, Eldred BSC, Kevan BM, Liau LM, Nghiemphu PL, Cloughesy TF, Lai A.(second PCR products) were ligated with plasmid vector using the TA cloning kit (New England BioLabs, Cat#E1203S), used to transform DH5α competent E. coli cells (Invitrogen, Cat#18258012) by standard methods; clonal

(11) Salicylic acid and RNA interference mediate antiviral immunity of plant stem cellsProceedings of the National Academy of SciencesOctober 17, 2023Marco Incarbone, Gabriele Bradamante, Florian Pruckner, Tobias Wegscheider, Wilfried Rozhon, Vu Nguyen, Ruben Gutzat, Zsuzsanna Mérai, Thomas Lendl, Stuart MacFarlane, et al.then ligating them into the pGGA-F GreenGate vectors or into the Golden Gate-ready pMiniT™2.0 (NEB #E1203S). Sequences containing BsaI cut sites (such as AtRDR1) were divided into several entry vectors, where

(12) Identification of Genes Associated with Liver Metastasis in Pancreatic Cancer Reveals PCSK6 as a Crucial MediatorCancers (Basel)December 30, 2022Hang He, Shuman Zhang, Hai Yang, Pengyan Xu, Isabella Kutschick, Susanne Pfeffer, Nathalie Britzen-Laurent, Robert Grützmann, Deliang Fu, Christian Pilarskyfollowed by ligation of the inserted DNA and vector using NEB PCR Cloning Kits (New England Biolabs, E1203S, Frankfurt, Germany). The generated vectors in above step were transformed into Competent E. coli (New

(13) Genome-Wide CRISPR Screening Identifies DCK and CCNL1 as Genes That Contribute to Gemcitabine Resistance in Pancreatic CancerCancersJune 27, 2022Yang H, Liu B, Liu D, Yang Z, Zhang S, Xu P, Xing Y, Kutschick I, Pfeffer S, Britzen-Laurent N, et al.and cloned PCR fragments into pMiniT 2.0 using an NEB PCR Cloning Kit (New England Biolabs, cat. # E1203S). We sequenced plasmid DNA through Eurofins Genomics. The sequencing PCR primers used in this study

(14) HIV-1 Employs Multiple Mechanisms To Resist Cas9/Single Guide RNA Targeting the Viral Primer Binding SiteJournal of VirologyOctober 15, 2018Zhen Wang, Wenzhou Wang, Ya Cheng Cui, Qinghua Pan, Weijun Zhu, Patrick Gendron, Fei Guo, Shan Cen, Michael Witcher, Chen LiangOneTaq one-step RT-PCR kit (catalog number E5315S; NEB) and cloned using the NEB PCR cloning kit (catalog number E1203S; NEB). On average, 40 or more DNA clones for each RT-PCR product were prepared and sequenced by MCLAB

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NEB PCR Cloning Kit (Without Competent Cells)

NEB PCR Cloning Kit (Without Competent Cells) from New England Biolabs

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New England Biolabs

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NEB PCR Cloning Kit (Without Competent Cells) from New England Biolabs

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