Monarch RNA Cleanup Kit (10 g) T2030S from New England Biolabs

Product Specs
ItemMonarch RNA Cleanup Kit (10 g)
CompanyNew England Biolabs
Price $58.00 Inquire
Catalog NumberT2030S
Quantity10 preps
ApplicationsDNA Manipulation
Temperature25 C

Add to Compare List

New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Cocaine remodels m6A RNA-dependent signaling to drive locomotor plasticity in Drosophila melanogasterFront Cell NeurosciApril 15, 2026Ana Filošević Vujnović, Nina Milotić, Bobana Samardžija, Marko Rubinić, Rozi Andretić Waldowski, Alessia SoldanoFragmented RNA was purified either by ethanol precipitation or using the Monarch RNA Clean-up Kit (NEB #T2030). For ethanol precipitation, RNA was mixed with 3 M sodium acetate (pH 5.2) and ethanol, incubated overnight

(2) Comparative analysis of flavivirus sfRNA dynamics and secondary structurebioRxivApril 8, 2026Camden R. Bair, David VanInsberghe, Anice C. Lowen, Graeme L. Conn, Christopher J. Neufeldt°C. DNase-treated RNA was then purified using the Monarch Spin RNA Cleanup kit (New England Biolabs, T2030L). RNA was eluted in 13 μl of nuclease-free water. RNA (10 μl) was then combined with 1 μl of 2 μM reverse-transcription

(3) Herpesvirus-encoded lncRNA targets host splicing by interacting with splicing factorsPLOS PathogensMarch 26, 2026Yuan Hong, Ritu Shekhar, Sarah McMahon, Netanya Keil, Melody Baddoo, J. Monty Watkins, Hasmik Keshishian, Caroline Stanclift, Steven A. Carr, Mathias Munschauer, et al.TRIzoL(Invitrogen). Each RNA was treated with DNaseI and re-purified using the Monarch Spin RNA Cleanup Kit (NEB #T2030). RT-qPCR was performed as mentioned above to detect ALT expression. Ribosomal RNA was depleted from

(4) Bacterial Schlafen proteins mediate phage defenceNature MicrobiologyMarch 9, 2026Veronica Perez Taboada, Yimo Wu, Riley Cassidy, Kirill E. Medvedev, Luuk Loeff, Anna Nemudraia, Artem Nemudryiinhibitor 1 U µl−1 (NEB)) for 1 h at 37 °C. Next, RNA was purified with the Monarch RNA Cleanup kit (NEB, T2030L). PNK-treated RNA samples were polyadenylated using E. coli Poly(A) polymerase (NEB, M0276S) in a reaction

(5) Three-dimensional genome reorganization foreshadows zygotic genome activation in DrosophilaNature GeneticsFebruary 24, 2026Noura Maziak, Yuchen Zhang, Fabian Groll, Haley E. Brown, Alla Madich, Yadwinder Kaur, Melissa M. Harrison, Jian Zhou, Juan M. Vaquerizasseparation and purified from the aqueous layer using the NEB Monarch RNA Cleanup Kit (NEB, cat. no. T2030). All samples showed RNA integrity no. >7 on a Bioanalyzer. Libraries were prepared with the Watchmaker

(6) Development of a non-invasive diagnostic method for pathogenic RNA viruses using sebum wiped from the cat’s body surfaceScientific ReportsFebruary 18, 2026Yuri V Fukushima, Namiko Saito, Hirohisa Mekata, Akatsuki Saitoperformed using the Monarch® Spin RNA Cleanup Kit (10 µg; New England Biolabs, Ipswich, MA, USA; Cat# T2030S). In brief, 200 µL Buffer BX and 300 µL ethanol were added to 100 µL RNA solution and mixed by pipetting.

(7) High-throughput analyses of a reconstituted diversity-generating retroelement identify intrinsic and extrinsic determinants of diversificationPLoS GenetFebruary 5, 2026Irem Unlu, Marina K. Smiley, Vladimir Potapov, Yoan Renoux-Martin, Zhi-Yi Sun, Hoong Chuin LimM0303) according to manufacturer’s recommendations and purified using Monarch Spin RNA Cleanup Kit (NEB, T2030). RT and qPCR reactions were then performed as described above with specific primers for TR-RNA only

(8) LINC01963 promotes pancreatic ductal adenocarcinoma proliferation via METTL3/IGF2BP2 axis-mediated m⁶A modification of c-MycJournal of Experimental & Clinical Cancer ResearchFebruary 4, 2026Qixian Liu, Ruiyu Li, Bohan Liu, Hangqi Liu, Xuqing Shi, Xianglin Yin, Xinping Ju, Sichong Zhang, Jun Wang, Yuhan Zhang, et al.NEBNext® Magnesium RNA Fragmentation Module (NEB, Cat# E6150), Monarch® RNA Cleanup Kit (NEB, Cat# T2030) and EpiMark® N6-Methyladenosine Enrichment Kit (NEB, Cat# E1610S) following the manufacturer’s protocols.

(9) Camsap2a regulates actomyosin flow and Rab5ab-mediated macropinocytosis in the yolk cell during zebrafish epibolyDevelopmentFebruary 1, 2026Haoyu Wan, Sifa Quibria, Ernest Iu, Sirma Damla User, Sergey V. Plotnikov, Ashley E. E. Bruceprobe was purified using NucAway Spin Columns (Ambion, AM10070) or the Monarch RNA Clean Up Kit (NEB, T2030L), following the manufacturers’ instructions. For campsap2a, a fragment from 618-1724 bp of the coding

(10) Methionine metabolism and the NOP2 methyltransferase are essential for MYC-Driven liver tumorigenesisbioRxivFebruary 1, 2026Sensen Lin, Charles Berdan, Moriah Sandy, Xinjun Lu, Vijay Ramani, Daniel K. Nomura, Xin Chen, Joyce V Lee, Andrei Gogaphosphorylation was catalyzed with T4 polynucleotide kinase. Enzymes were removed by RNA purification kit (NEB T2030). Seq libraries were constructed with NEBNext Multiplex Small RNA Library Prep Set for Illumina, according

(11) Periovulatory neurohormone dynamics reveal an association between secretoneurin and GnRH across the mouse estrous cycleFront Endocrinol (Lausanne)January 23, 2026Chunyu Lu, Di Peng, Kevin B. Smith, Chinelo Uju, Suraj Unniappan, Paula Duarte-Guterman, Nafissa Ismail, Vance L. TrudeauResidual DNA was removed using DNase I (NEB, Cat# M0303S) followed by Monarch RNA Cleanup Kit (NEB, Cat# T2030L). RNA quality was assessed using the Qubit RNA HS Assay (Thermo Fisher, Cat# Q32852) and RNA ScreenTape

(12) Genome-wide profiling of RNA 2’-O-methylation in neurons and identification of orphan snoRNA targetsResearch SquareJanuary 20, 2026Gordon Carmichael, Xuan Ye, Yaling Liu, Yinzhou Zhu, Saran Alshawi, Brittany Elliott, Christopher Holley, Jean-Denis Beaudoin, Brenton R. Graveleyincubation, RNA purification was carried out using the Monarch Spin RNA Cleanup kit (New England Biolabs, #T2030) according to the manufacturer’s instructions, and the RNA product was eluted in 20 μL of nuclease-free

(13) Complexoform-restricted covalent TRMT112 ligands that allosterically agonize METTL5Nature Chemical BiologyJanuary 8, 2026F. Wieland Goetzke, Steffen M. Bernard, Cheng-Wei Ju, Jonathan Pollock, Kristen E. DeMeester, Jacob Gross, Gabriel M. Simon, Chuan He, Bruno Melillo, Benjamin F. Cravattabove. After the time points, the liquid was subjected to a Monarch Spin RNA cleanup kit (10 μg) (NEB, T2030L), eluted with 17 μl of water and digested overnight as described above. The nucleosides were analyzed

(14) Single-Cell Transcriptomics on PRPF31-Mutated Retinal Organoids Reveal Early Müller Glial Activation and Progressive Photoreceptor DegenerationBiomedicinesDecember 24, 2025Alessandro Bellapianta, Jingjing Qi, Michele Giugliano, Sara Ouaidat, Rana El Rawas, Matthias Bolz, Ahmad Saltimanufacturer’s protocol. RNA cleanup procedure was performed using the Monarch® RNA Cleanup Kit Protocol (T2030L; New England Biolabs, Frankfurt am Main, Germany), and the RNA was eluted in 20 µL of RNase-free water

(15) Epstein-Barr Virus Promotes Gastric Cancer Progression by Modulating m6A-Dependent YTHDF1-TSC22D1 AxisMicroorganismsDecember 11, 2025Yea Rim An, Jaehun Jung, Kyeong Min Kwon, Jun Yeob Kim, Min-Hyeok Lee, Ju Yeon Lee, Minho Lee, Suk Kyeong LeeNew England Biolabs, Ipswich, MA, USA) and subsequently purified using the Monarch RNA Cleanup Kit (#T2030, New England Biolabs). Library preparation was performed using the TruSeq Stranded Total RNA Library Prep

(16) FluoTag-EMSA: a fast and accessible quantitative method to assess RNA-binding specificity using 3'-tagged hybrid duplexesFront Mol BiosciDecember 10, 2025Benjamin Rothé, Daniel B. Constaminhibitor (Roche, 03 335 402 001)DNase I (Roche, 04 716 728 001)Monarch® Spin RNA Cleanup Kit (NEB, T2030) For agarose gel electrophoresis.AgaPure™ Agarose LE (Canvax, CANAG006)10× TAE (Tris-Acetate-EDTA) buffer:

(17) Single-base m 6 A epitranscriptomics reveals novel HIV-1 host interaction targets in primary CD4 + T cellsJournal of VirologyNovember 25, 2025Siyu Huang, Yutao Zhao, Stacia Phillips, Julia E. Warrick, Michael G. Kearse, Chuan He, Li Wu(202003, Synaptic Systems) or rabbit IgG (cat, vendor) was used for RNA-IP. The Monarch RNA Cleanup Kit (T2030S, New England Biolabs) was used to purify the enriched RNA. RT-qPCR was conducted to detect target gene

(18) Persistent and Dose-Dependent Neural and Metabolic Gene Expression Changes Induced by Transient Citalopram Exposure in Zebrafish EmbryosInternational Journal of Molecular SciencesNovember 22, 2025Ryan J. North, Gwendolyn Cooper, Lucas Mears, Brian Bothner, Mensur Dlakić, Christa S. Merzdorfsamples were subsequently purified using the Monarch RNA Cleanup kit (New England Biolabs, Cat. No. T2030L, Ipswich, MA, USA). RNA quality was assessed with a Nanodrop spectrophotometer and an Agilent Bioanalyzer

(19) Cappable-Seq reveals the transcriptional landscape of stress responses in the bacterial endosymbiont WolbachiaMicrob GenomOctober 27, 2025Youseuf Suliman, Zhiru Li, Amit Sinha, Philip D. Dyer, Catherine S. Hartley, Laurence Ettwiller, Alistair C. Darby, Clotilde K. Carlow, Benjamin L. Makepeacefor 1 h. Samples underwent RNA purification using the Monarch RNA clean-up kit (NEB product number T2030S), modified to increase the washing step to a total of five times to ensure removal of free DTB-GTP;

(20) Bacterial Schlafens mediate anti-phage defensebioRxivJuly 30, 2025Veronica Perez Taboada, Yimo Wu, Riley Cassidy, Kirill E. Medvedev, Luuk Loeff, Anna Nemudraia, Artem Nemudryiinhibitor 1U/ μL (NEB)) for 1 hour at 37 °C. Next, RNA was purified with the Monarch RNA Cleanup Kit (NEB, T2030L). PNK-treated RNA samples were polyadenylated using E. coli Poly(A) polymerase (NEB, M0276S) in a reaction

Be the First to Write a Review!

Monarch RNA Cleanup Kit (10 g)

Monarch RNA Cleanup Kit (10 g) from New England Biolabs

Description

New England Biolabs

Citations (93)

Reviews

Monarch RNA Cleanup Kit (10 g) from New England Biolabs

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map