HiScribe T7 ARCA mRNA Kit E2065S from New England Biolabs

Product Specs
ItemHiScribe T7 ARCA mRNA Kit
CompanyNew England Biolabs
Price $344.00 Inquire
Catalog NumberE2065S
Quantity20 reactions
ApplicationsFunctional Genomics
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Engineered mRNA Backbones for Gene Expression in Human T-CellsMolecular Therapy Nucleic AcidsJune 16, 2026Gilad Gibor, Neve Tzvi, Amilia Meir, Hiba Abu-Hariri, Anat Shemer, Shai Kilim, Sophie Abelian, Ortal Harush, Orit Itzhaki, Ronnie Shapira-Frommer, et al.In vitro transcription and nucleotide modification IVT was performed using the HiScribe T7 ARCA mRNA Kit (NEB #E2065S) enriched with pseudouridine-UTP (Ψ-UTP). Each 20 μL reaction contained 10 μL ARCA/NTP mix, 2.5 μL Ψ-UTP,

(2) Maternal obesity disrupts epigenetic reprogramming via peroxisomal-dependent phospholipid-methyl uncoupling during zygotic genome activationNature CommunicationsMarch 18, 2026Xiao-Guohui Zhang, Lin-Li Yang, Xie Feng, Yu-Wei Zhang, Lei Guo, Sun-Xing Huang, Tie-Gang Meng, Pei-Ying Li, Lei-Ning Chen, Rui-Bao Su, et al.Vazyme), and in vitro transcription of capped mRNA was performed using the HiScribe T7 ARCA mRNA Kit (E2065S, NEB, USA). Subsequently, the HyperScribe Poly(A) Tailing Kit (K1053, APExBIO, USA) was used to add

(3) Genetic landscape of human oocyte/embryo defectsCell GenomicsJanuary 14, 2026Biaobang Chen, Weijie Wang, Juanzi Shi, Xiaoxi Sun, Yichun Guan, Guimin Hao, Junli Zhao, Jian Mu, Zhihua Zhang, Fangzhou Xu, et al.restriction enzymes,New England BioLabs,Cat# R3552S HiScribe T7 ARCA mRNA Kit,New England BioLabs,Cat# E2065S RNeasy MinElute Cleanup Kit,Qiagen,Cat# 217084 DMEM,Gibco,Cat# MA0212 Penicillin/streptomyci,Gibco,Cat#

(4) CRISPR-Cas9 HDR optimization: RAD52, denatured, and 5′-modified DNA templates in knock-in mice generationiScienceNovember 21, 2025Boris V. Skryabin, Daniela A. Braun, Helena Kaiser, Leonid Gubar, Birte Seeger, Tasneem Khanam, Anja Stegemann, Hermann Pavenstädt, Timofey S. Rozhdestvenskycommercial assays,Critical commercial assays,Critical commercial assays ,, HiScribe® T7 ARCA mRNA Kit,NEB,E2065S Qiaquick extraction kit,Qiagen,Cat # 28704 ,, Experimental models: Organisms/strains,Experimental models

(5) Ubiquitin-Specific Protease 6 mRNA Lipid Nanoparticles Ignite Antitumor Immunity and Suppress Tumorigenesis in Ewing SarcomaMolecular cancer therapeuticsNovember 3, 2025Henrich IC, Billingsley MM, Jain K, Mondal S, Quick LN, Young R, Nguyen N, Oliveira AM, Blobel GA, Mitchell MJ, et al.mRNA was in vitro transcribed using HiScribe T7 ARCA mRNA Kit (with or without tailing; NEB #E2060S or E2065S, respectively) for EGFP or USP6 mRNA, respectively. Modified nucleotides pseudo-uracil (TriLink #N-1019)

(6) Mevalonate metabolites boost aged oocyte quality through prenylation of small GTPasesNature AgingAugust 26, 2025Chuanming Liu, Huidan Zhang, Jialian Mao, Sainan Zhang, Xiao Tian, Yibing Zhu, Changjiang Wang, Junshun Fang, Huijie Pan, Nannan Kang, et al.template with AscI (NEB, R0558V), capped mRNA was synthesized using a HiScribe T7 ARCA mRNA Kit (NEB, E2065S), column-cleaned with NucleoSpin RNA Clean-up XS (Macherey-Nagel, 740903) and dephosphorylated for uncapped

(7) Deficient of glycosylation site in the envelop protein attenuated Zika virus replication in mosquito cellsFrontiers in MicrobiologyAugust 25, 2025Wen-Jing Wang, Zi-Han Wang, Jing Li, Sai-Ya Ma, Mei He, Meng-Xuan Liu, Yu-Fei Zhan, Feng Jin, Guosheng Qu, Chunhong Yin, et al.In vitro-transcribed viral RNA was prepared using a HiScribe® T7 ARCA mRNA Kit (New England Biolabs, E2065S) and purified by phenol-chloroform extraction (P.C.I). The viral mRNA was then transfected into Vero-E6

(8) GORK K+ channel structure and gating vital to informing stomatal engineeringNat CommunFebruary 25, 2025Xue Zhang, William Carroll, Thu Binh-Anh Nguyen, Thanh-Hao Nguyen, Zhao Yang, Miaolian Ma, Xiaowei Huang, Adrian Hills, Hui Guo, Rucha Karnik, et al.mRNAs (cRNAs) were generated from linearised oocyte-expression plasmids (HiScribe® T7 ARCA mRNA Kit, E2065S, New England Biolabs) and were injected at 25 ng/oocyte. Recordings were carried out 72 h after injection

(9) Chromosome architecture and low cohesion bias acrocentric chromosomes towards aneuploidy during mammalian meiosisNature CommunicationsDecember 23, 2024Eirini Bellou, Agata P. Zielinska, Eike Urs Mönnich, Nina Schweizer, Antonio Z. Politi, Antonina Wellecke, Claus Sibold, Andreas Tandler-Schneider, Melina SchuhEllenberg) were also used. All mRNAs in this study were synthesised using HiScribe T7 ARCA mRNA kit (NEB #E2065S) according to the manufacturer’s protocol and quantified using Qubit RNA HS Assay Kit (Thermo Fisher Scientific

(10) VPS34 Governs Oocyte Developmental Competence by Regulating Mito/Autophagy: A Novel Insight into the Significance of RAB7 Activity and Its Subcellular LocationAdvanced ScienceNovember 1, 2024Wenwen Liu, Kehan Wang, Yuting Lin, Lu Wang, Xin Jin, Yuexin Qiu, Wenya Sun, Ling Zhang, Yan Sun, Xiaowei Dou, et al.Kit (GeneMark, DP034P‐300). Then mRNAs were created and tailed using HiScribe T7 ARCA mRNA Kit (NEB, E2065S), purified by a RNA Clean & Concentrator‐25 Kit (Zymo Research, R1017), and stored at −80 °C for future

(11) Antibodies targeting the Crimean-Congo Hemorrhagic Fever Virus nucleoprotein protect via TRIM21Nature CommunicationsOctober 25, 2024Shanna S. Leventhal, Thomas Bisom, Dean Clift, Deepashri Rao, Kimberly Meade-White, Carl Shaia, Justin Murray, Evan A. Mihalakakos, Troy Hinkley, Steven J. Reynolds, et al.plasmids were linearized and 5′-capped mRNA was synthesized with T7 polymerase (NEB HiScribeT7 ARCA kit; E2065S) according to manufacturer’s instructions. The sequences of the plasmids are available on request. mRNA

(12) Potassium channel TASK-5 forms functional heterodimers with TASK-1 and TASK-3 to break its silenceNature CommunicationsAugust 30, 2024Susanne Rinné, Florian Schick, Kirsty Vowinkel, Sven Schütte, Cornelius Krasel, Silke Kauferstein, Martin K.-H. Schäfer, Aytug K. Kiper, Thomas Müller, Niels DecherNheI and cRNA was synthesized using the HiScribe T7 ARCA mRNA Kit (New England Biolabs, catalog no E2065S). cDNA of the α1 receptor was linearized with BamHI and cRNA synthesis was done with the mMESSAGE mMACHINE™

(13) The maintenance of oocytes in the mammalian ovary involves extreme protein longevityNature Cell BiologyJuly 1, 2024Katarina Harasimov, Rebecca L. Gorry, Luisa M. Welp, Sarah Mae Penir, Yehor Horokhovskyi, Shiya Cheng, Katsuyoshi Takaoka, Alexandra Stützer, Ann-Sophie Frombach, Ana Lisa Taylor Tavares, et al.pGEMHE-Ub(G76V)-mClover3-T2A-mScarlet was linearized with PacI and then transcribed in vitro using the HiScribe T7 ARCA mRNA Kit (NEB; E2065S). The transcribed mRNA was further purified using the RNeasy Mini Kit (Qiagen; 74104). To test whether

(14) Hybridization led to a rewired pluripotency network in the allotetraploid Xenopus laevisElifeOctober 3, 2023Wesley A Phelps, Matthew D Hurton, Taylor N Ayers, Anne E Carlson, Joel C Rosenbaum, Miler T Leeassembly (NEB #E2621) for pou5f3.3 and BamHI/XbaI for sox3. IVT was done using NEB HiScribe T7 ARCA kit (#E2065S) on NotI-linearized plasmid for 2 hr at 37 °C, then treated with 5 U of TURBO DNaseI (Invitrogen #AM2238)

(15) Hybridization led to a rewired pluripotency network in the allotetraploid Xenopus laeviseLifeOctober 3, 2023Wesley A Phelps, Matthew D Hurton, Taylor N Ayers, Anne E Carlson, Joel C Rosenbaum, Miler T Leeassembly (NEB #E2621) for pou5f3.3 and BamHI/XbaI for sox3. IVT was done using NEB HiScribe T7 ARCA kit (#E2065S) on NotI-linearized plasmid for 2 hr at 37 °C, then treated with 5 U of TURBO DNaseI (Invitrogen #AM2238)

(16) Maintenance of a host-specific minority mutation in the West Nile virus NS3iScienceAugust 18, 2023Haley S. Caldwell, Lili Kuo, Janice D. Pata, Alan P. Dupuis, Jamie J. Arnold, Calvin Yeager, Jessica Stout, Cheri A. Koetzner, Anne F. Payne, Sean M. Bialosuknia, et al.mRNA transfection reagent,Polyplus,Cat# 101000005 HiScribe® T7 ARCA mRNA Kit,New England Biolabs,Cat# E2065S ,, Experimental models: Cell lines,Experimental models: Cell lines,Experimental models: Cell lines ,, Culex

(17) The PRMT5/WDR77 complex restricts hepatitis E virus replicationPLoS pathogensJune 1, 2023Ju X, Yu Y, Ren W, Dong L, Meng X, Deng H, Nan Y, Ding Q.plasmid templates were transcribed in vitro with HiScribe T7 antireverse cap analog (ARCA) mRNA kit (E2065S, NEB) according to the manufacturer’s instructions. Viral RNA was transfected into HepG2C3A cells or

(18) Drag-and-drop genome insertion of large sequences without double-strand DNA cleavage using CRISPR-directed integrasesNature biotechnologyApril 1, 2023Yarnall MTN, Ioannidi EI, Schmitt-Ulms C, Krajeski RN, Lim J, Villiger L, Zhou W, Jiang K, Garushyants SK, Roberts N, et al.Bxb1 mRNA (NLS-Bxb1) were transcribed and poly-A tailed using the HiScribe™ T7 ARCA mRNA Kit (NEB, E2065S) with 50% supplement of 5-Methyl-CTP and Pseudo-UTP (Jena Biosciences), following the manufacturer’s

(19) eIF4A1-dependent mRNAs employ purine-rich 5'UTR sequences to activate localised eIF4A1-unwinding through eIF4A1-multimerisation to facilitate translationNucleic acids researchFebruary 28, 2023Schmidt T, Dabrowska A, Waldron JA, Hodge K, Koulouras G, Gabrielsen M, Munro J, Tack DC, Harris G, McGhee E, et al.UK), GFP (ab13970, Abcam UK), vinculin (ab129002, Abcam UK). Kits: HiScribe™ T7 ARCA mRNA Kit (NEB E2065S), HiScribe™ T7 mRNA Kit with CleanCap® Reagent AG (NEB E2080S), Rabbit Reticulocyte Lysate (Promega

(20) Cytokinesis During the First Division of a Mouse EmbryoFrontiers in Cell and Developmental BiologyFebruary 1, 2022Xiao-Yan Fan, Xing-Hua Wang, Feng-Yun Xie, Jun-Yu Ma, Xiang-Hong Ou, Shi-Ming Luoobtained the mRNA by utilizing an RNA transcription kit (HiScribeTM T7 ARCA mRNA Kit (with tailing), E2065S, New England Biolabs, United States). The GFP-labeled actin and RFP-labeled lysosome plasmids were gifts

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HiScribe T7 ARCA mRNA Kit

HiScribe T7 ARCA mRNA Kit from New England Biolabs

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HiScribe T7 ARCA mRNA Kit from New England Biolabs

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