NEBridgeGolden Gate Assembly Kit (BsaI-HFv2) E1601S from New England Biolabs

Product Specs
ItemNEBridgeGolden Gate Assembly Kit (BsaI-HFv2)
CompanyNew England Biolabs
Price $164.00 Inquire
Catalog NumberE1601S
Quantity20 reactions
ApplicationsDNA Manipulation
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Global distribution of isoprenoid quinones across BacteriamSystemsJuly 21, 2026Sophie-Carole Chobert, Suraj Kanwar, Olivier Lerouxel, Nelle Varoquaux, Julie Michaud, Ludovic Pelosi, Fabien Pierrel, Sophie S. Abbyreading frame, and a terminator) were assembled using the NEBridge Golden Gate Assembly Kit (BsaI-HFv2, #E1601, New England BioLabs), in accordance with the manufacturer’s protocol and as described in reference

(2) Parallel gene amplification by Cas9 nickase for generating functionally heterogeneous cell populationsCell Reports MethodsJuly 20, 2026Hiroaki Takesue, Satoshi Okada, Takashi ItoHMW DNA Extraction Kit for Tissue,NEB,Cat# T3060L NEB Golden Gate Assembly Kit (BsaI-HF v2),NEB,Cat# E1601L NEBuilder HiFi DNA Assembly Master Mix,NEB,Cat# E2621L Ligation Sequencing Kit,Oxford Nanopore Technologies,SQK-LSK114 Native

(3) Exploring bet-hedging in Salmonella enterica serovar Typhimurium with a dual reporter strainmSystemsJune 23, 2026Michelle I. Gerber, Murugesan Sivaranjani, Kyle Vincent, Samantha Jelfimow, Dakoda J. Herman, Melissa B. Palmer, Chinenye R. Nnajide, Haley Sanderson, Jessica Sparrow, Brandon M. Waddell, et al.were synthesized as five DNA fragments (IDT Canada), and Golden Gate assembly (New England Biolabs, # E1601S) was used to construct them into the pGGA recipient plasmid (Fig. S7). The dual reporter constructs

(4) Dissecting serum polyclonal antibody escape to SARS-CoV-2 variants by deep mutational learningCell Reports MethodsJune 15, 2026Danielle Shlesinger, Viktor Sadilek, Mason Minot, Evangelos Stamkopoulos, Thomas Bikias, Raphael Kuhn, Andreas Agrafiotis, Joseph M. Taft, Alexander Yermanos, Sai.T. Reddythe RBD, using the NEBridge Golden Gate assembly kit according to manufacturer’s instructions (NEB #E1601). The assembled product was transformed into E. coli DH5α Mix & Go! Competent Cells competent cells

(5) Loop extrusion by cohesin plays a role in enhancer-activated gene expression early in differentiationNature CommunicationsMay 26, 2026Rosa J. Stolper, Felice H. Tsang, Emily Georgiades, Lars L. P. Hanssen, Edward A. J. Tunnacliffe, Damien J. Downes, Caroline L. Harrold, Jim R. Hughes, Robert A. Beagrie, Benjamin Davies, et al.Prof. Benjamin Davies group) between the pair of BsaI sites with the NEB Golden Gate Assembly Kit (NEB #E1601). The pROSA-TV2 was designed to contain the 1.4 and 1.2 homology arms of the inserted site and a hygromycin

(6) The apical polar ring is essential during the blood stage of Plasmodium falciparumNature MicrobiologyMay 14, 2026Pratima Gurung, Peter S. Back, Ilzat Ali, Gabrielle M. Hernandez, Jeffrey D. Dvorinassembled from five PCR-amplified DNA fragments via Golden Gate (GG) assembly with the NEB BsaI-HFv2 kit (E1601L, NEB), following the manufacturer’s protocol. The fragments included the following: (1) the 5′ UTR region,

(7) Single-nucleus epigenomic dysregulation unmasks genetic risk-associated neurodegenerative glia statesNature CommunicationsMay 14, 2026Xia Han, Gregory M. Rosenberg, Vivianne M. Kisling, Tao Zhang, Chia-Yi Lee, Ashvin Ravi, Mikhail Melnik, Tina Bilousova, Salvatore Spina, Alissa L. Nana, et al.and Blasticidin resistance via Golden Gate Assembly following to the manufacturer’s protocol (NEB #E1601). Plasmids were sequence verified prior to lentiviral packaging, and 300 uL aliquots were frozen at

(8) PEX11 mediates intralumenal vesicle formation in peroxisomesNat CommunApril 21, 2026Nathan E. Tharp, Chelsea An, James Hwang, Nayeli S. Shad, Zachary J. Wright, Bonnie BartelBsaI sites of the pAGM55261 vector (Addgene 153210)45 using Golden Gate assembly (New England Biolabs, E1601) to yield Cas9-expressing vectors with gRNA sequences flanked by a U6 promoter and gRNA scaffold. Constructs

(9) High-throughput screening of FRET-based proteins using a hyperspectral microcapillary arrayiScienceApril 17, 2026Khushank Singhal, Thomas M. Baer, Benjamin D. Allen, Melik C. Demirelproteins" ,, Ultrapure water,Milli-Q,Equation 7000 NEBridge Golden Gate Assembly Kit,New England Biolabs,E1601S NEBuilder HiFi DNA Assembly Master Mix,New England Biolabs,E2621S BamHI-HF,New England Biolabs,R3136S PstI-HF,New England Biolabs

(10) Physiological architecture and evolutionary origins of cellular adaptabilitybioRxivApril 15, 2026Annisa Dea, Yongqing Lan, Benjamin A. Doran, Asif Ali, Maya G. Igarashi, Lucas Dyer, Valeryia Aksianiuk, David Pincus, Arjun S. Ramansynthesized by IDT and inserted into pCRCT using the Golden Gate Assembly Kit BsaI-HF® v2 (NEB, Cat. # E1601S). All engineered mutations were verified by Sanger sequencing. Competitive fitness assays Growth competitions

(11) Transmembrane proteins mediate basal complex assembly and individual daughter cell formation in malaria parasitesScience AdvancesFebruary 20, 2026Peter S. Back, Erinn Wagner, Paula Montero Llopis, Ramon G. de Oliveira, Marco Mottinelli, Lori Ferrins, Jeffrey D. DvorinGolden Gate assemblies were performed with NEBridge Golden Gate Assembly Kit (BsaI-HFv2) (catalog no. E1601). All Gibson assemblies were performed with NEBuilder HiFi DNA Assembly Cloning Kit (catalog no. E5520).

(12) Engineering electron conduits in bacteria for selective biointerfacing and enhanced energy transferiScienceFebruary 20, 2026Alexander R. Kelly, Lorenzo Travaglini, Dominic J. Gloverplasmid purification,QIAGEN,Cat#12125 Gibson assembly master mix,NEB,Cat#E2611S NEBridge Golden Gate assembly kit (BsaI-HF v2),NEB,Cat#E1601S Q5 High-Fidelity 2X master mix,NEB,Cat#M0492S OneTaq 2X master mix with standard buffer,NEB,Cat#M0482S ,, Recombinant

(13) Scalable TCR synthesis and screening enables antigen reactivity mapping in vitiligoImmunityFebruary 10, 2026Stephanie A. Gaglione, Rachit S. Mukkamala, Chirag Krishna, Blake E. Smith, Marc H. Wadsworth, Scott A. Jelinsky, Caleb R. Perez, Laura Schmidt-Hong, Erica L. Katz, Kyle J. Gellatly, et al.Gate Assembly Kit (BsmBI-v2),NEB,Cat#E1602 NEBridge Golden Gate Assembly Kit (BsaI-HF-v2),NEB,Cat#E1601 BsaI-HF-v2,NEB,Cat#R3733 BsmBI-v2,NEB,Cat#R0739 BbsI-HF,NEB,Cat#R3539 SapI,NEB,Cat#R0569 FastDigest XbaI

(14) uSort-M: Scalable isolation of user-defined sequences from diverse pooled librariesbioRxivJanuary 15, 2026Micah B. Olivas, Patrick J. Almhjell, Jack D. Shanahan, Polly M. FordyceSupporting Information) via a Golden Gate reaction using NEBridge BsaI-HFv2 Golden Gate master mix (NEB E1601S). Assembly reactions were used directly to transform NEB 5-alpha chemically competent E. coli (NEB C2987H).

(15) The essential function of the apical polar ring during the blood stage of Plasmodium falciparumbioRxivJanuary 10, 2026Pratima Gurung, Peter S. Back, Ilzat Ali, Gabrielle M. Hernandez, Jeffrey D. Dvorinassembled from five PCR-amplified DNA fragments via Golden Gate (GG) assembly with the NEB BsaI-HFv2 kit (E1601L, NEB), following the manufacturer’s protocol. The fragments included: (1) the 5’ UTR region, amplified

(16) Assessment of the phi6 lysis system using genetic complementation and heterologous expression in Escherichia coli and Pseudomonas syringaeFrontiers in MicrobiologyJanuary 7, 2026Devyn Del Curto, Micaela Zamora, Greyson Lasley, Rabia Khan, Katlyn Montalbano, J. Bryce Ricken, Jesse Cahillacids (aa). The pJLC140 shuttle vector was constructed using the Golden Gate assembly kit (BsaI, NEB #E1601L) following the manufacturer’s recommended protocols. Assembly involved combining three synthetic DNA

(17) Sustained in situ protein production and release in the mammalian gut by an engineered bacteriophageNature BiotechnologyJanuary 1, 2026Zachary R. Baker, Yao Zhang, Haiyan Zhang, Hollyn C. Franklin, Priscila B. S. Serpa, Teresa Southard, Liwu Li, Bryan B. HsuMolecular cloning Genetic constructs were designed in Benchling and assembled using Golden Gate Assembly73 (NEB, Catalog # E1601L) with assistance from the NEB Golden Gate Assembly Tool. Plasmids were transformed into DH5α cells (NEB,

(18) Transcription factors instruct DNA methylation patterns in plant reproductive tissuesNature Cell BiologyNovember 21, 2025Guanghui Xu, Yuhan Chen, Laura M. Martins, En Li, Fuxi Wang, Tulio Magana, Junlin Ruan, Julie A. Lawegg-cell-driven zCas9 gene, using Golden Gate cloning per the manufacturer’s instructions (NEB, cat. no. E1601). Primers used to make the CRISPR constructs are listed in Supplementary Data 11. The constructs were

(19) Systematic genome-wide mapping of host determinants of bacteriophage infectivityCell SystemsNovember 19, 2025Chutikarn Chitboonthavisuk, Cody Martin, Phil Huss, Jason M. Peters, Karthik Anantharaman, Srivatsan Raman(Roche KK5005) was utilized. The New England Biosciences (NEB) Golden Gate Assembly Kit (BsaI-HF v2, E1601L) and the Gibson Assembly Protocol (NEB E5510) were employed, utilizing our in-house-prepared Gibson Assembly Master Mix

(20) A modular high-throughput approach for advancing synthetic biology in the chloroplast of ChlamydomonasNature PlantsNovember 3, 2025René M. Inckemann, Tanguy Chotel, Michael Burgis, Cedric K. Brinkmann, Laura Andreas, Jessica Baumann, Priyati Sharma, Melanie Klose, James Barrett, Fabian Ries, et al.the NEBridge Golden Gate Assembly Kit from New England Biolabs for both BsaI and BsmBI enzymes (NEB #E1601 and NEB #E1602). The reaction mixtures, prepared without specifying volumes, included a vector at 10

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NEBridgeGolden Gate Assembly Kit (BsaI-HFv2)

NEBridgeGolden Gate Assembly Kit (BsaI-HFv2) from New England Biolabs

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NEBridgeGolden Gate Assembly Kit (BsaI-HFv2) from New England Biolabs

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