Luna Universal One-Step RT-qPCR Kit E3005S from New England Biolabs

Product Specs
ItemLuna Universal One-Step RT-qPCR Kit
CompanyNew England Biolabs
Price $263.00 Inquire
Catalog NumberE3005S
Quantity200 reactions
ApplicationsDNA Analysis, RT-qPCR
Temperature-20 C

Add to Compare List

New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Rho2-dependent cell wall remodeling boosts the fungistatic activity of manogepix against Aspergillus fumigatusThe Cell SurfaceJune 1, 2026Sean Brazil, Isabel Kopp-Fouquet, Samuel Zen Kai Yap, Michael Carty, Johannes WagenerRT-qPCR was performed using 1 μg of template RNA per well and the LUNA Universal One Step RT-qPCR Kit (E3005S; New England Biolabs). Expression of gwt1 was normalized to tubA (β-tubulin; AFUA_1G10910) and analysed

(2) Incorporation of genome-bound cellular proteins into HIV-1 particles regulates viral infectionCell RepApril 7, 2026Manuel Garcia-Moreno, Azman Embarc-Buh, Robin Truman, Marko Noerenberg, Louisa Iselin, Honglin Chen, Caroline E. Lenz, Jeffrey Y. Lee, Kate Dicker, Snehith Dyavari Shetty, et al.sample dilution was quantified using the Luna Universal One-step RT-qPCR kit (New England Biolabs, #E3005S) with specific primers against the 5′ end of HIV-1 gRNA (Key Resources Table). RT-qPCRs were performed

(3) Electromagnetic exposure changes human Schwann cells motility and transcriptomic profile of hearing-loss related genesiScienceMarch 20, 2026Tasnim Mohamed, Alessandra Colciago, Alessandro Faroni, Adam J. Reid, Giulio Ferrero, Valerio Magnaghi"Cell Signaling Technology, Milan, Italy",Cat# 6813 Luna® One-Step RT-qPCR Kit,"New England Biolabs, Ipswich, MA, USA",Cat#E3005S ,, Deposited data,Deposited data,Deposited data RNA-seq files,NCBI GEO,GSE322769 (https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE322769) Code

(4) GFER Represents a Target for Dual Disruption of Redox Homeostasis and Reactivation of the Immune Response in Pancreatic AdenocarcinomaCancer ResearchMarch 2, 2026Ziheng Chen, I-Lin Ho, Jintan Liu, Melinda Soeung, Er-Yen Yen, Sara Sainani, Charles A. Dyke, Rutvi Shah, Hyuk Jean Kwon, Guangchun Han, et al.extracts and cytosolic fractions was detected by qPCR using the Luna Universal One-Step RT-qPCR Kit (NEB #E3005S/L) with specific primer sequences. The qPCR primer sequences are for human, MT-ND1(mtDNA), forward primer

(5) Host-directed broad-spectrum immunotherapeutic strategy for respiratory infections: Heat-killed Caulobacter crescentus (HKCC) as an innate-immune based biotherapeutic/postbioticPLOS PathogensFebruary 25, 2026Shanika Werellagama, Raj S. Patel, Nancy Gupta, Satish Vedi, Diana Duque, Jegarubee Bavananthasivam, Rakesh Kumar, Anh Tran, Babita AgrawalTotal viral genomic RNA was quantified with the RT-qPCR kit (Luna Universal One-Step RT-qPCR; Cat. E3005S; New England Biolabs). RNA quantification and analysis were carried out using an Applied Biosystems

(6) The mechanism of cell-cycle-dependent proteasomal degradation of archaeal ESCRT-III homolog CdvB in SulfolobusThe EMBO JournalJanuary 9, 2026Yin-Wei Kuo, Jovan Traparić, Sherman Foo, Buzz BaumDNase,Invitrogen,AM2238 RNeasy Mini Kit,Qiagen,74104 Luna Universal One-Step RT-qPCR Kit,New England Biolabs,E3005S Software,Software,Software Fiji,"Schindelin et al, 2012",fiji.sc Flowjo v10,BD Biosciences,www.flowjo.com GraphPad

(7) Membrane potential mediates the cellular response to mechanical pressureCellJanuary 8, 2026Avik Mukherjee, Yanqing Huang, Jens Elgeti, Seungeun Oh, Jose G. Abreu, Leander Ammar, Anjali R. Neliat, Janik Schüttler, Dan-Dan Su, Christophe Dupre, et al.addition of IPTG to the culture medium. Knockdown efficiency was confirmed by RT-qPCR (using NEB Luna, cat#E3005S) (Figure S2A) after induction by 10 mM IPTG (Millipore-Sigma, cat#16758) for 5 days. After establishing

(8) HyperdCas12a-based multiplexed genetic regulation in Candida albicansNucleic Acids ResDecember 11, 2025Nicholas C Gervais, Ruby K J Rogers, Madeleine R Robin, Rebecca S Shapiroused immediately. RT-qPCR was performed with the Luna® Universal One-Step RT-qPCR Kit from NEB (cat. E3005S) using an input of 200–300 ng of RNA (exact amount was kept consistent within each experiment). RNA

(9) Formins and Arp2/3 Reciprocally Regulate Contact Guidance on Aligned Collagen FibrilsCellular and Molecular BioengineeringDecember 1, 2025Azarnoosh Foroozandehfar, Shayan Tohidi, Shafayet Ahmed Siddiqui, Fred Rogers Namanda, Michael Forrester, Eric Cochran, Ian C. Schneideralignment. Reverse Transcription Quantitative PCR (RT-qPCR) We used a Luna Universal one-step RT-qPCR kit (E3005S, New England BioLabs, Ipswich, MA). According to the manufacturer’s protocol, 0.8 μg of total RNA was

(10) Butyrate Enhances Antimicrobial Defence in Chicken Macrophages Through Reactive Oxygen Species Generation and Autophagy ActivationCellsNovember 6, 2025James R. G. Adams, Faisal R. Anjum, Jai W. Mehat, Roberto M. La Ragione, Shahriar BehboudiLuna® Universal One-Step Reaction Mix, 0.5 µL Luna® WarmStart® RT Enzyme Mix (New England Biolabs, E3005S, Ipswich, MA, USA), 0.5 µL forward primer, 0.5 µL reverse primer, and 2 µL nuclease free water. qPCR

(11) Evaluation of Salivary HLA-DR4 and MMP-8 Levels Along with Porphyromonas gingivalis in Periodontitis Patients with Rheumatoid ArthritisF1000ResOctober 30, 2025Shaymaa Abdulkareem Nori, Batool Hassan Al-Ghurabi, Nik Nairan AbdullahKit = EK716715 Presto™ Mini gDNA Bacteria Kit = GBB100/101 Luna ® Universal qPCR Master Mix = NEB #E3005S/L Study population This study included 80 subjects aged between 30 and 55 years. All participants

(12) Rho2 regulates granulocyte-triggered stress adaptation and cell wall remodeling in Aspergillus fumigatusMicrobiological ResearchOctober 1, 2025Dominik Ruf, Kristina Striegler, Sean Brazil, Hesham Elsaman, Kalpana Singh, Mathieu Lepas, Liyanage D. Fernando, Victor Brantl, Patricia Heß, Karl Dichtl, et al.Waltham, MA, USA) using 1 μg of template RNA per well and the LUNA Universal One Step RT-qPCR Kit (E3005S; New England Biolabs). Expression of rho2 was normalized to tubA (β-tubulin; AFUA_1G10910) and analyzed

(13) MitoScribe single-cell molecular recorder logs graded signaling dynamics into mitochondrial DNAbioRxivSeptember 11, 2025Linhan Wang, Nikolaos Poulis, Deepak Srivastava, Seth L ShipmanAnalysis of hypoxia-downstream gene expression was performed with Luna Universal One-Step RT-qPCR (NEB, E3005S) using the primers listed in Supplementary Table 2. Data was normalized to β-actin abundance, and mRNA

(14) Dopaminergic Modulation of Short-Term Associative Memory in Caenorhabditis elegansJ NeurochemAugust 19, 2025Anna McMillen, Caitlin Minervini, Renee Green, Michaela E. Johnson, Radwan Ansaar, Yee Lian ChewTotal RNA Kit (NEB #T2010S) and converted to cDNA using the Luna Universal One‐Step RT‐qPCR Kit (NEB #E3005S). Two forms of the dop‐2 cDNA sequence were cloned: one aligned to the DOP‐2S sequence published in

(15) H3K4me2 distinguishes a distinct class of enhancers during the maternal-to-zygotic transitionPLoS BiolJuly 10, 2025Matthew D. Hurton, Jennifer M. Miller, Miler T. LeeqRT-PCR, 40 ng RNA per embryo was used as template for the Luna Universal One-Step RT-qPCR Kit (NEB #E3005S) with three technical replicates per embryo per primer pair. qRT-PCR was carried out on a QuantStudio

(16) Metformin reduces the competitive advantage of Dnmt3aR878H HSPCsNatureJune 12, 2025Mohsen Hosseini, Veronique Voisin, Ali Chegini, Angelica Varesi, Severine Cathelin, Dhanoop Manikoth Ayyathan, Alex C. H. Liu, Yitong Yang, Vivian Wang, Abdula Maher, et al.spectrophotometer. RT–qPCR analysis was performed using the Luna Universal One-Step RT–qPCR kit (NEB, E3005S) on a Bio-Rad CFX Touch Real-Time PCR Detection System. Gene expression was normalized to mouse Actb

(17) High-throughput screening of human genetic variants by pooled prime editingCell GenomicsApril 9, 2025Michael Herger, Christina M. Kajba, Megan Buckley, Ana Cunha, Molly Strom, Gregory M. Findlayvariant (p.Thr335=) and c.1119−12C>G was subjected to RT-qPCR using Luna Universal One-Step RT-qPCR Kit (#E3005S, NEB) in technical duplicates with custom primer sets designed using the Primer3 webtool (Table S9).

(18) Transfer RNA acetylation regulates in vivo mammalian stress signalingSci AdvMarch 21, 2025Supuni Thalalla Gamage, Roxane Khoogar, Shereen Howpay Manage, Judey T. DaRos, McKenna C. Crawford, Joe Georgeson, Bogdan V. Polevoda, Chelsea Sanders, Kendall A. Lee, Kellie D. Nance, et al.described above. RT-qPCR was performed using the Luna Universal One-step RT-qPCR kit (New England Biolabs, E3005S) using a LightCycler 480 II PCR instrument (Roche). Real-time instrument was programmed with the following

(19) The Salmonella pathogenicity island 1-encoded small RNA InvR mediates post-transcriptional feedback control of the activator HilA in SalmonellaJournal of BacteriologyMarch 20, 2025Yutong Hou, Kyungsub Kim, Fatih Cakar, Yekaterina A. Golubeva, James M. Slauch, Carin K. Vanderpoolphenol–chloroform and ethanol precipitation. Luna Universal One-Step RT-qPCR kit (New England Biolabs, E3005S) was used for the cDNA synthesis and PCR amplification reaction. Specifically, 100 ng of total RNA from

(20) TET2 deficiency increases the competitive advantage of hematopoietic stem and progenitor cells through upregulation of thrombopoietin receptor signalingNat CommunMarch 10, 2025Yitong Yang, Severine Cathelin, Alex C. H. Liu, Amit Subedi, Abdula Maher, Mohsen Hosseini, Dhanoop Manikoth Ayyathan, Robert Vanner, Steven M. Chantranscription and quantitative PCR were performed using the Luna® Universal One-step RT-qPCR kit (NEB, #E3005S) and the Bio-Rad CFX touch real-time PCR detection system. The primers used are listed in Supplementary

Be the First to Write a Review!

Luna Universal One-Step RT-qPCR Kit

Luna Universal One-Step RT-qPCR Kit from New England Biolabs

Description

New England Biolabs

Citations (75)

Reviews

Luna Universal One-Step RT-qPCR Kit from New England Biolabs

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map