NEBNextSingle Cell/Low Input RNA Library Prep Kit for Illumina E6420L from New England Biolabs

Product Specs
ItemNEBNextSingle Cell/Low Input RNA Library Prep Kit for Illumina
CompanyNew England Biolabs
Price $4,104.00 Inquire
Catalog NumberE6420L
Quantity96 reactions
ApplicationsDNA Analysis, Library Prep, NGS
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Chemokine receptor expression defines a trajectory from monocytes to mature macrophages in the lungJ Leukoc BiolMay 13, 2026Heather Mathie, Laura Medina-Ruiz, Fabian Schuette, Heba Halawa, Zuzanna Pocalun, Elise Pitmon, John Cole, Marieke Pingen, Gerard J Grahamthe NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina (New England Biolabs; cat. no. E6420L). Samples were sequenced using paired-end sequencing on the NextSeq2000 sequencing platform (Illumina)

(2) Systematic evaluation of 24 extraction and library preparation combinations for metagenomic sequencing of SARS-CoV-2 in salivabioRxivApril 24, 2026Kenin Qian, Varada Abhyankar, Dahlia Keo, Payton Zarceno, Traci Toy, Eleazar Eskin, Valerie A. Arboledalibraries. We used the NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina (New England Biolabs, E6420L) along with the NEBNext Multiplex Oligos for Illumina (New England Biolabs, E6440L) according to the

(3) Parabrachial CGRP Neurons Regulate Opioid ReinforcementbioRxivApril 8, 2026Lauren L. Bystrom, Alexander V. Margetts, Nicole M. Kujas, Florence M Bourgain-Guglielmetti, Elizabeth P Marinov, Luis M. Tuestaprepared using NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina (New England Biolabs, E6420L) according to the manufacturer’s instructions. Sequencing was performed on a Novaseq 6000 (Illumina)

(4) Haploid Mutation Mapping Identifies a Homoeologous Non‐Reciprocal Translocation Linked to Reduced Fibre and Enhanced Protein in Brassica napusPlant Biotechnology JournalJanuary 29, 2026Morgan W. Kirzinger, Sarika Saini, Andrea T. Todd, Ushan Alahakoon, Kevin C. Koh, Justin B. Nichol, HaiYing Yuan, Kevin Fengler, Victor Llaca, Dustin Cram, et al.using the NEBNext Single Cell/Low Input RNA library Prep Kit for Illumina (New England Biolabs, Cat# E6420L) with the following modifications. For inputs of RNA with 2000 or more cells/embryo, 10 PCR cycles for

(5) Functional evidence for early origin of tactile acuity in the vertebrate somatosensory systemCurrent BiologyOctober 6, 2025Ke Tan, Lorraine F. Hillgen-Santa, Morven Graham, Yaping Li, Xinran Liu, Song Pang, Elena O. Gracheva, Sviatoslav N. Bagriantsevthe NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina (New England Biolabs, cat. no. E6420L) and sequenced on the NovaSeq platform (paired-end, 150 bp) at the Yale Center for Genome Analysis,

(6) Impaired hematopoiesis and embryonic lethality at midgestation of mice lacking both lipid transfer proteins VPS13A and VPS13CPLOS BiologySeptember 16, 2025Peng Xu, Rubia Isler Mancuso, Marianna Leonzino, Caroline J. Zeiss, Diane S. Krause, Pietro De Camillifor library preparation. Using the NEBNext Single Cell/Low Input RNA Library Prep Kit from Illumina (E6420L), a normalized RNA input between 200 pg and 50 ng was used to generate cDNAs with the template switching

(7) Defect in hematopoiesis and embryonic lethality at midgestation of Vps13a/Vps13c double knockout micebioRxivMay 16, 2025Peng Xu, Rubia Isler Mancuso, Marianna Leonzino, Caroline J. Zeiss, Diane S. Krause, Pietro De Camillifor library preparation. Using the NEBNext Single Cell/Low Input RNA Library Prep Kit from Illumina (E6420L), a normalized RNA input between 200pg-50ng was used to generate cDNAs with the template switching method.

(8) Drosophila AHR limits tumor growth and stem cell proliferation in the intestineWellcome Open ResApril 25, 2025Minghua Tsai, Jiawei Sun, Cyrille Alexandre, Michael Shapiro, Adrien Franchet, Ying Li, Alex P. Gould, Jean-Paul Vincent, Brigitta Stockinger, Nicola Laura DinySingle Cell/Low Input RNA Library Prep Kit for Illumina® Instruction Manual Version 5.0_5/20, NEB #E6420L). Samples were normalized to 1ng total RNA material per library in 8μl of nuclease-free water. RNA samples

(9) Local treatment of HVJ-E with T cell costimulatory molecule stimulation elicits systemic anti-tumor effectsMol Ther OncolDecember 19, 2024Ishibashi A, Li Y, Hisatomi Y, Ohta N, Uegaki Y, Tanemura A, Ohashi R, Kitamura K, Saga K, Yoshimura Y, et al.T cells were constructed using the NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina (E6420L, New England Biolabs) following the manufacturer’s instructions as described previously.38 Two biological

(10) Simple aneuploidy evades p53 surveillance and promotes niche factor-independent growth in human intestinal organoidsMolecular Biology of the CellAugust 1, 2024Blake A. Johnson, Albert Z. Liu, Tianhao Bi, Yi Dong, Taibo Li, Dingjingyu Zhou, Akshay Narkar, Yufei Wu, Sean X. Sun, Tatianna C. Larman, et al.,New England Biolabs,Catalogue# E5530S NEBNext Single Cell/Low Input RNA Library Prep Kit,New England Biolabs,Catalogue# E6420L NEBNext Multiplex Oligos,New England Biolabs,Catalogue# E6440S Click-iT EdU Alexa Fluor 488,Invitrogen,Catalogue#

(11) Simple aneuploidy evades p53 surveillance and promotes niche factor-independent growth in human intestinal organoidsMol Biol CellJuly 22, 2024Blake A. Johnson, Albert Z. Liu, Tianhao Bi, Yi Dong, Taibo Li, Dingjingyu Zhou, Akshay Narkar, Yufei Wu, Sean X. Sun, Tatianna C. Larman, et al.,New England Biolabs,Catalogue# E5530S NEBNext Single Cell/Low Input RNA Library Prep Kit,New England Biolabs,Catalogue# E6420L NEBNext Multiplex Oligos,New England Biolabs,Catalogue# E6440S Click-iT EdU Alexa Fluor 488,Invitrogen,Catalogue#

(12) The genomic and cellular basis of biosynthetic innovation in rove beetlesCellJuly 11, 2024Sheila A. Kitchen, Thomas H. Naragon, Adrian Brückner, Mark S. Ladinsky, Sofia A. Quinodoz, Jean M. Badroos, Joani W. Viliunas, Yuriko Kishi, Julian M. Wagner, David R. Miller, et al.kit,Illumina,Cat# RS-122-2001 NEBNext Single Cell/Low Input RNA Library Prep Kit,New England Biolabs,Cat# E6420L MEGAscript T7 Transcription Kit,Thermo Fisher Scientific,Cat #AMB13345 Deposited Data,Deposited Data,Deposited

(13) Transendothelial Migration of Human B Cells: Chemokine versus AntigenThe Journal of ImmunologySeptember 15, 2023Vivian Wang, Jordan S. Pober, Thomas D. Maneslibrary prep. RNA Seq Library Prep: Using the NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina (E6420L) and a normalized RNA input between 200pg-50ng a template switching method is used to generate cDNAs.

(14) The RNA binding proteins TIA1 and TIAL1 promote Mcl1 mRNA translation to protect germinal center responses from apoptosisCellular & Molecular ImmunologyJuly 20, 2023Ines C. Osma-Garcia, Mailys Mouysset, Dunja Capitan-Sobrino, Yann Aubert, Martin Turner, Manuel D. Diaz-MuñozNEBNext® Single Cell/Low Input RNA Library Prep Kit for Illumina® (New England Biolabs [NEB], Cat No. E6420L). NEBNext® Multiplex Oligos for Illumina® (Dual Index Primers Set 1 or 2, NEB, Cat No. E7780S) were

(15) Resolving the origins of secretory products and anthelmintic responses in a human parasitic nematode at single-cell resolutionElifeJune 15, 2023Clair R Henthorn, Paul M Airs, Emma K Neumann, Mostafa Zamanianprepared using the NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina (NEB, version 3.0, #E6420L) and NEBNext Multiplex Oligos for Illumina (Index Primers Set 1, NEB, #E6440G), followed by 2 × 150

(16) Resolving the origins of secretory products and anthelmintic responses in a human parasitic nematode at single-cell resolutioneLifeJune 15, 2023Clair R Henthorn, Paul M Airs, Emma K Neumann, Mostafa Zamanianprepared using the NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina (NEB, version 3.0, #E6420L) and NEBNext Multiplex Oligos for Illumina (Index Primers Set 1, NEB, #E6440G), followed by 2 × 150

(17) In vivo expansion of a CD9(+) decidual-like NK cell subset following autologous hematopoietic stem cell transplantationiScienceOctober 21, 2022Orrantia A, Vázquez-De Luis E, Astarloa-Pando G, Terrén I, Amarilla-Irusta A, Polanco-Alonso D, González C, Uranga A, Carrascosa T, Mateos-Mazón JJ, et al.Kit,Qiagen,Cat# 74034 NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina,New England Biolabs,Cat# E6420L Super-Script IV VILO Master Mix with ezDNase enzyme,Invitrogen,Cat#11766050 Brilliant III Ultra-Fast SYBR Green qPCR Master Mix

(18) Spatial transcriptomics reveals antiparasitic targets associated with essential behaviors in the human parasite Brugia malayiPLOS PathogensApril 7, 2022Paul M. Airs et al.2.11.11 of the NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina (NEB, version 3.0, #E6420L) using NEBNext Multiplex Oligos for Illumina (Index Primers Set 1, NEB, #E6440G) and sequenced on an

(19) Cells of the human intestinal tract mapped across space and timeNatureSeptember 1, 2021Elmentaite R, Kumasaka N, Roberts K, Fleming A, Dann E, King HW, Kleshchevnikov V, Dabrowska M, Pritchard S, Bolt L, et al.Smart-seq2 Plate-based scRNA-seq was performed with the NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina (E6420L; New England Biolabs). Total cell fractions from dissociated gut sections of donors BRC2033–2034, F67,

(20) Distinct microbial and immune niches of the human colonNature immunologyMarch 1, 2020James KR, Gomes T, Elmentaite R, Kumar N, Gulliver EL, King HW, Stares MD, Bareham BR, Ferdinand JR, Petrova VN, et al.with the NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina (New England Biolabs Inc, E6420L). Cells from donor 364b were snap frozen in 10% DMSO in 90% BSA. Cells were thawed rapidly in a 37°C

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NEBNextSingle Cell/Low Input RNA Library Prep Kit for Illumina

NEBNextSingle Cell/Low Input RNA Library Prep Kit for Illumina from New England Biolabs

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New England Biolabs

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NEBNextSingle Cell/Low Input RNA Library Prep Kit for Illumina from New England Biolabs

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