Fig 1: ANXA1-mediated PTHrP and p-Smad2 expression in SCLC cells. (A) Western blotting, (B) reverse transcription-quantitative PCR and (C) ELISA of PTHrP expression in SCLC cells. (D) Western blot analysis of Smad2 and p-Smad2 in SCLC cells treated with TGF-ß. *P<0.05 vs. TGF-ß (-). ANXA1, annexin A1; SCLC, small cell lung cancer; PTHrP, parathyroid hormone-related protein; p, phosphorylated; NC, negative control; sh, short hairpin.