Fig 1: Monocyte-derived macrophages from patients with active CD have reduced selenoprotein expression despite selenium sufficiency.Monocyte-derived macrophages from patients with active CD or individuals in disease remission have reduced selective selenoprotein expression as determined by qPCR (A) and immunoblotting (B; densitometry (n = 3) and a representative blot)) as compared to cells from healthy volunteers. Selenium status was assessed by selenium intake using 24 h food recalls (C), serum SelenoP concentration (D), and serum GPx activity (E) by ELISA and enzymatic activity assay, respectively. Data are means ± SEM (*p < 0.05, **p < 0.005; SCLY, Selenocysteine lyase).
Fig 2: Macrophages from patients with active CD have reduced selenoprotein mRNA expression in situ.Published scRNAseq data (SCP1884) was reanalyzed examining colonic macrophage mRNA expression in healthy biopsies and biopsies from patients with CD. (A) UMAP representation of the macrophage clusters. (B) Relative abundance of each cluster between groups. Top differentially expression pathways between scRNAseq data from inflamed biopsies from patients with CD compared with healthy biopsies (C). The same dataset was used to assess selenoprotein mRNA expression of selenoprotein P (SEPP1), glutathione peroxidase (GPx) 1 and GPx4 in macrophages from healthy biopsies and inflamed and non-inflamed biopsies from patients with CD (D) including in the different sub-populations (E, F). (Heal Healthy, Infl Inflamed, NonI Non-inflamed).
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