Fig 1: Receiver operating characteristic curves (ROC) of plasma homocysteine, ROCK2, and Vimentin for distinguishing PEXS and PEXG from control subjects. (A-C) Hcy showed excellent diagnostic performance with AUCs of 0.98 (95% CI: 0.95–1.00) for Control vs. PEX (PEXS + PEXG), 0.98 (95% CI: 0.95–1.00) for Control vs. PEXS, and 0.97 (95% CI: 0.92–1.00) for Control vs. PEXG. Optimal cut-off values were > 15.09 µmol/L, > 15.19 µmol/L, and > 21.40 µmol/L, respectively. (D-F) ROC analysis of ROCK2 yielded AUCs of 0.77 (95% CI: 0.66–0.88), 0.74 (95% CI: 0.60–0.88), and 0.80 (95% CI: 0.68–0.93) for Control vs. PEX (PEXS + PEXG), PEXS, and PEXG, with corresponding cut-offs of > 375.1 pg/mL, > 378.5 pg/mL, and > 353.1 pg/mL. (G-I) VIM showed AUCs of 0.94 (95% CI: 0.88–0.99), 0.89 (95% CI: 0.79–0.99), and 0.99 (95% CI: 0.97–1.00) for Control vs. PEX (PEXS + PEXG), PEXS, and PEXG, with optimal cut-offs of > 484.60 pg/mL, > 467.30 pg/mL, and > 486.30 pg/mL, respectively
Fig 2: SP1 as a key transcriptional regulator in Pseudoexfoliation. (A) Transcription factor enrichment using TRRUST and ChEA3 identified SP1 as a top-scoring regulator of PEX-associated genes (B–C). In silico prediction of putative SP1 binding sites performed within the proximal promoter regions (− 1500 bp upstream of the TSS) of VIM and ROCK2 using the UCSC Genome Browser and JASPAR (motif MA0079.3)
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