Fig 1: Optimization of AGE-induced late glycation modeling conditions and positive control selection. (A) Effects of different concentrations of AGEs on HFF-1 cell viability (CCK-8 assay). (B) Effects of different concentrations of AGEs on type Col I content (ELISA assay). (C) Effects of different concentrations of alagebrium chloride (ALT-711) on HFF-1 cell viability (CCK-8 assay). (D) Effects of different concentrations of ALT-711 on ELN content in 200 μg/mL AGEs-induced HFF-1 cells (ELISA assay). Data are presented as mean ± SD. Data presented are from one experiment with 6 technical replicates for CCK-8 assays and 3 technical replicates for ELISA assays. * p < 0.05, vs. the NC group; # p < 0.05 vs. the model group.