Fig 1: Regulation of Nur77-NF-κB signaling by DCA.a PLS-DA plot of the transcriptome from sheep colon epithelium (n = 5). b Volcano plot of the transcriptome from sheep colon epithelium (n = 5). c GSEA plot of the NF-κB signaling pathway in the transcriptome from sheep colon epithelium (n = 5). d, e Protein expression levels of Nur77 and p65 in sheep colon epithelium (n = 3). f After 48 h of culture, Caco-2 cells were co-treated with DCA at varying concentrations (10, 25, 50, 100, and 200 μM) and TNF-α (10 ng/mL) for 12 h. g Cell viability of Caco-2 cells treated with different concentrations of DCA (n = 8). h, i The concentrations of IL-1β and IL-6 in the supernatant of Caco-2 cells (n = 8). j, k Protein expression levels of Nur77 and p65 in Caco-2 cells following 25 μM DCA treatment (n = 3). l, m Protein expression levels of Nur77 and p65 in Caco-2 cells following OA inhibitor intervention (n = 3). n Concentrations of IL-1β and IL-6 in the supernatant of Caco-2 cells following OA inhibitor intervention (n = 8). Data were presented as mean ± SEM. Statistical analysis of data was performed using a two-tailed Student’s t test or a two-tailed Wilcoxon rank-sum test (group number = 2). One-way ANOVA or the non-parametric Kruskal-Wallis test was used for statistical analysis of data (group number >2).