Fig 1: Serum levels of parathyroid, calcitonin, testosterone and 17-beta estradiol hormones and prostaglandin E2 after 28 days of starting the experiment in different rat groups. Data are expressed as mean ± SE. abcd Columns not sharing a common superscript letter are significantly different (p < 0.001) and the highest value was represented by the letter (a), same letters indicate no differences. PTH-parathyroid hormone; CT-calcitonin; PGE2- prostaglandin E2.
Fig 2: METTL3 inhibition facilitated VC in CKD rats.A Scheme of the construction of the CKD-associated VC rat model. B Serum levels of P, Ca, and PTH in control or CKD or CKD + SAH rats (n = 6). Data are shown as mean ± standard deviation. a, means the difference between the control group; b, means the difference between the CKD group. Representative images of Alizarin red S staining (C) and von Kossa staining (D) to reveal the calcium deposition in the thoracic and abdominal aortas of control or CKD or CKD + SAH rats. E Calcium content quantification of aortas to detect mineralization of control or CKD or CKD + SAH rats (n = 3). F Representative immunofluorescence pictures and quantification of METTL3 and Runx2 in aortas of control or CKD or CKD + SAH rats (n = 3). G Representative blot images of METTL3, Runx2, and osteogenic reprogramming markers in aortas of control or CKD or CKD + SAH rats (n = 3). *p < 0.05, **p < 0.01, ***p < 0.001. Statistical significance between the groups was determined by one-way ANOVA. SAH S-Adenosylhomocysteine, P phosphate, Ca calcium, PTH parathyroid hormone.
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