Fig 1: TGF-β1, SMAD3, SMAD7 and CTGF mRNA expression levels. (A) TGF-β1, (B) SMAD3, (C) SMAD7 and (D) CTGF mRNA expression levels were analyzed in RLE-6TN cells treated with 200 nmol/l paraquat, 10 ng/ml tacrolimus and 5 µmol/lLY2109761 combinations using reverse transcription-quantitative PCR. *P<0.05 vs. β-actin; ΔP<0.05 vs. paraquat only. TGF-β1, transforming growth factor-β1; CTGF, connective tissue growth factor.
Fig 2: TGF-β1, SMAD3, SMAD7 and CTGF concentrations in RLE-6TN cell culture supernatants. Concentrations of (A) TGF-β1, (B) SMAD3, (C) SMAD7 and (D) CTGF were determined using ELISAs following 200 nmol/l paraquat, 10 ng/ml tacrolimus and 5 µmol/l LY2109761 treatment. *P<0.05 vs. untreated cells; ΔP<0.05 vs. paraquat only. TGF-β1, transforming growth factor-β1; CTGF, connective tissue growth factor.
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