Description
Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The Caspase-10 Fluorometric Assay Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence AEVD. The assay is based on detection of cleavage of substrate AEVD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). AEVD-AFC emits blue light (λmax = 400 nm); upon cleavage of the substrate by caspase-10 or related caspases, free AFC emits a yellow-green fluorescence (λmax = 505 nm), which can be quantified using a fluorometer or a fluorecence microtiter plate reader