Description
The most versatile reporter gene is the firefly luciferase. Recently there is steadily increasing use of other luciferases, such as Gaussia luciferase since these reporters are smaller and do not require the presence of ATP. The bioluminescent enzyme derived from the marine copepod Gaussia prince is efficiently secreted from mammalian cells upon expression. Gaussia luciferase is a 20 kDa protein which catalyzes coelenterazine oxidation by oxygen to produce light