Description
Although chromogenic assays of β-galactosidase activity (i.e. X-Gal) have found extensive use as do the application of the fluorogenic substrates (for example FDG) detection of lacZ activity using luminescence can give even greater levels of sensitivity. The sensitive Glo-Gal substrate is cleaved first by the β-galactosidase enzyme and then in the presence of ATP luciferase acts to produce red light output