Description
The JMJD2C Homogeneous Assay Kit is designed to measure activity of the JMJD2C for screening and profiling applications, using AlphaLISA technology. The key to the JMJD2C Homogeneous Assay Kit is a highly specific antibody that recognizes demethylated substrate. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, a sample containing JMJD2C enzyme is incubated with the biotinylated substrate. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts