E.coli HCP ELISA kit DEIABL481 from Creative Diagnostics

Product Specs
ItemE.coli HCP ELISA kit
CompanyCreative Diagnostics
Price Supplier Page View Company Product Page
Catalog NumberDEIABL481
Quantity96T
ReactivityE.coli
ApplicationsELISA
Components1. Clear 96-Well Strip Plate
Clear plastic strip-well microtiter plate coated with rabbit anti-E. coli HCP IgG. Can be used as individual strips.
2. E. coli Protein Standard (2430 ng/ml, 600 µl)
Concentrated E. coli proteins sufficient for generating a standard curve from 810 ng/ml to 1.1 ng/ml.
3. 5x Dilution Buffer (15 ml)
1x Dilution Buffer is used for dilution of Reporting Antibody and Streptavidin-HRP conjugate. 1x Dilution Buffer is used to dilute samples if necessary. The 10 ml of 5x concentrate should be diluted to 50 ml with 40 ml of milliQ water to achieve 1x Dilution Buffer.
4. 10x PBS-T (30 ml)
1x PBS-T is used for wash steps. The 25 ml of 10x concentrate should be diluted to 250 ml with 225 ml of milliQ water to achieve 1x PBS-T.
5. Reporting Antibody (135 µl/tube)
A biotin labeled rabbit polyclonal antibody specific for E. coli cell proteins. Immediately prior to the assay, dilute 120 µl into 12 ml of 1x Dilution Buffer.
6. Streptavidin-HRP Conjugate (4 µg/ml, 420 µl/tube)
A Streptavidin–Horse Radish Peroxidase conjugate in a stabilizing solution. Immediately prior to the assay, dilute 375 µl into 15 ml of 1x Dilution Buffer to give a 0.1 µg/ml working stock.
7. TMB Substrate (15 ml)
Use directly without dilution.
8. Stop Solution (15 ml)
A 1M solution of sulfuric acid. CAUSTIC. Use directly without dilution. The solution should not come in contact with skin or eyes. Take appropriate precautions when handling this reagent.
9. Plate Sealer

Add to Compare List

Creative Diagnostics

Phone: 1-631-624-4882 (USA)
44-161-818-6441 (Europe)

Email: info@creative-diagnostics.com

Company Profile

Website: https://www.creative-diagnostics.com/

Be the First to Write a Review!

E.coli HCP ELISA kit from Creative Diagnostics

Creative Diagnostics

Reviews

E.coli HCP ELISA kit from Creative Diagnostics

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map